IL-5R alpha 蛋白, Human (Biotinylated, HEK293, His-Avi)

IL-5R alpha protein is a cytokine receptor that is expressed on eosinophils, basophils and B cells and is involved in inflammation and immune regulation.IL-5R alpha Protein, Human (Biotinylated, HEK293, His-Avi) is expressed by HEK293 cells with His-Avi tags^[1][3].

IL-5R α 是一种 I 型细胞因子受体，由两条不同的多肽链 α 和 β 组成。α 链是一种穿膜糖蛋白，可特异性结合 IL-5，β 链可将低亲和力 IL-5R 转化为高亲和力 IL-5R，形成异二聚体受体复合物^[1]。
IL-5R α 与 IL-5 结合后可诱导多种细胞内信号通路的激活，包括 JAK/STAT、RAF/MAP、Ras-Raf-ERK 和磷脂酰肌醇 3-激酶^[2] .
IL-5R α 在嗜酸性粒细胞、嗜碱性粒细胞和 B 细胞上表达，影响细胞存活、凋亡、分化和趋化性^[3]。

Measured by its binding ability in a functional ELISA. When immobilized Human IL-5 His at 0.5 μg/mL (100μL/Well) , can bind biotinylated Human IL-5 R alpha His and the EC₅₀ is 0.12 ug/mL.

Human

HEK293

C-Avi;C-His

Q01344-1 (D21-E335)

3568  [NCBI]

Approximately 38.7 kDa

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. S Takaki, et al. A critical cytoplasmic domain of the interleukin-5 (IL-5) receptor alpha chain and its function in IL-5-mediated growth signal transduction. Mol Cell Biol. 1994 Nov;14(11):7404-14.  [Content Brief]

  [2]. R P de Groot, et al. Regulation of proliferation, differentiation and survival by the IL-3/IL-5/GM-CSF receptor family. Cell Signal. 1998 Oct;10(9):619-29.  [Content Brief]

  [3]. S C Barry, et al. Analysis of interleukin 5 receptors on murine eosinophils: a comparison with receptors on B13 cells. Cytokine. 1991 Jul;3(4):339-45.  [Content Brief]