IL-6 蛋白, Canine

IL-6 is a multifunctional cytokine that binds to IL6R and forms a complex with IL6ST/gp130 to initiate intracellular signaling. It induces "classical signaling" and "trans signaling" by interacting with membrane-bound IL6R or soluble IL6R. IL-6 Protein, Canine is the recombinant canine-derived IL-6 protein, expressed by E. coli , with tag free.

IL-6蛋白是一种多功能细胞因子，在免疫、组织再生和代谢中发挥广泛的生物学功能。与其受体 IL6R 结合后，该复合物与信号亚基 IL6ST/gp130 结合，启动细胞内 IL-6 信号传导途径。膜结合的 IL6R 和 IL6ST 之间的相互作用触发“经典信号传导”，而 IL-6 和可溶性 IL6R 与 IL6ST 的结合则刺激“反式信号传导”。此外，当发射细胞上的膜结合 IL6:IL6R 复合物激活邻近接收细胞上的 IL6ST 受体时，就会发生“簇信号传导”。IL-6 是急性期反应的有效诱导剂，有助于宿主在感染和组织损伤期间的防御；然而，过度的 IL-6 合成与疾病病理有关。在先天免疫反应中，巨噬细胞和树突状细胞等骨髓细胞通过感染或损伤部位的 Toll 样受体 (TLR) 识别病原体后合成 IL-6。此外，IL-6 在适应性免疫反应中至关重要，对于 B 细胞分化为免疫球蛋白分泌细胞至关重要，并且在 CD4(+) T 细胞亚群的分化中发挥重要作用，包括滤泡辅助性 T (Tfh) 的发育生发中心形成和驱动初始 CD4(+) T 细胞向 Th17 谱系所需的细胞。IL-6 还被证明对于骨髓瘤细胞增殖和浆母细胞存活是必需的。

Measured in a cell proliferation assay using TF-1 cells. The ED₅₀ for this effect is 15.5 ng /mL, corresponding to a specific activity is 6.45×10⁴ units/mg.

Canine

E. coli

Tag Free

P41323 (T23-M207)

403985  [NCBI]

TPGPLAGDSKDDATSNSLPLTSANKVEELIKYILGKISALRKEMCDKFNKCEDSKEALAENNLHLPKLEGKDGCFQSGFNQETCLTRITTGLVEFQLHLNILQNNYEGDKENVKSVHMSTKILVQMLKSKVKNQDEVTTPDPTTDASLQAILQSQDECVKHTTIHLILRSLEDFLQFSLRAVRIM

Approximately 20.8 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4, 5% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.