IL-6 蛋白, Equine

IL-6 protein is a crucial cytokine in immunity, tissue regeneration and metabolism. It binds to IL6R and forms a complex that binds to IL6ST/gp130, triggering intracellular IL6 signaling. Membrane-bound IL6:IL6R complexes induce "cluster signaling" that activates IL6ST receptors on neighboring cells. IL-6 Protein, Equine is the recombinant equine-derived IL-6 protein, expressed by E. coli , with tag free.

IL-6是一种多功能细胞因子，在免疫、组织再生和代谢方面发挥多种生物学作用。在与其受体 IL6R 结合后，该复合物与信号亚基 IL6ST/gp130 结合，启动细胞内 IL6 信号通路。当 IL6 与膜结合的 IL6R 结合时，这种相互作用可以引发“经典信号传导”，或者当 IL6 和可溶性 IL6R 与 IL6ST 结合时，这种相互作用可以引发“反式信号传导”。此外，当发射细胞上的 IL6:IL6R 复合物激活邻近接收细胞上的 IL6ST 受体时，就会发生“簇信号传导”。IL-6 是急性期反应的重要诱导剂，在感染和组织损伤期间快速产生，用于宿主防御。然而，过量的 IL-6 合成与多种疾病病理有关。在先天免疫反应中，包括巨噬细胞和树突状细胞在内的骨髓细胞通过感染部位或组织损伤处的 Toll 样受体 (TLR) 识别病原体后合成 IL-6。在适应性免疫反应中，IL-6 对于 B 细胞分化为免疫球蛋白分泌细胞、滤泡辅助 T (Tfh) 细胞发育以及驱动幼稚 CD4(+) T 细胞向 Th17 谱系发展至关重要。此外，IL-6 在骨髓瘤细胞增殖和浆母细胞存活中发挥重要作用。

Measured in a cell proliferation assay using TF-1 cells. The ED₅₀ for this effect is 0.6786 ng/mL, corresponding to a specific activity is 1.474×10⁶ units/mg.

Equine

E. coli

Tag Free

Q95181 (F26-M208)

100034196  [NCBI]

FPTPLPLGEDETTSNGPLLTTADKTKQHIKYILGKISALKNEMCNNFSKCENSKEVLAENNLNLPKMAEKDGCFQSGFNQETCLMKITTGLSEFQIYLEYLQNEFKGEKENIKTMQISTKVLVQILMQKMKNPEVTTPDPTAKSSLLAKLHSQNEWLKNTTTHLILRSLEDFLQFSLRAVRIM

Approximately 21 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 6.5, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.