IL-6 蛋白, Mouse (His)

IL-6 protein is involved in various functions such as immunity, tissue regeneration and metabolism.IL-6 Protein, Mouse (His) is the recombinant mouse-derived IL-6 protein, expressed by E.coli , with N-6*His labeled tag.

IL-6 蛋白是一种多功能细胞因子，具有涵盖免疫、组织再生和代谢等一系列生物功能，复杂地参与多方面的信号网络。与其受体 IL6R 结合后，所得复合物与信号亚基 IL6ST/gp130 结合，启动 IL6 信号通路。IL-6 系统表现出多种信号传导模式：与膜结合的 IL6R 和 IL6ST 相互作用时的“经典信号传导”、与可溶性 IL6R 的“反式信号传导”以及涉及细胞间通讯的“簇信号传导”。从功能上讲，IL-6 可作为急性期反应的有效诱导剂，在感染和组织损伤期间迅速动员宿主防御。在先天免疫反应中，巨噬细胞和树突状细胞等骨髓细胞通过 Toll 样受体合成 IL-6，以响应病原体识别。此外，IL-6 在适应性免疫反应中发挥着关键作用，对于 B 细胞分化至关重要，尤其是在免疫球蛋白分泌细胞的生成中。此外，IL-6 是驱动 CD4(+) T 细胞亚群分化的关键因素，对于滤泡辅助 T (Tfh) 细胞的发育和促进有效的抗体反应至关重要。它的作用延伸到与 IL21 协同诱导 Tfh 细胞，并控制骨髓瘤细胞的增殖和浆母细胞的存活。这种全面的功能强调了 IL-6 在免疫调节和体内平衡中的核心作用。

Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED₅₀ for this effect is ≤346.9 pg/mL, corresponding to a specific activity is ≥2.88×10⁶ units/mg.

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  Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED₅₀ for this effect is 284.5 pg/mL, corresponding to a specific activity is 3.51×10⁶ units/mg.

Mouse

E. coli

N-6*His

P08505 (F25-T211)

16193  [NCBI]

FPTSQVRRGDFTEDTTPNRPVYTTSQVGGLITHVLWEIVEMRKELCNGNSDCMNNDDALAENNLKLPEIQRNDGCYQTGYNQEICLLKISSGLLEYHSYLEYMKNNLKDNKKDKARVLQRDTETLIHIFNQEVKDLHKIVLPTPISNALLTDKLESQKEWLRTKTIQFILKSLEEFLKVTLRSTRQT

Approximately 24 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4 or 20 mM Sodium Acetate, pH 4.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.