IL-6 蛋白, Pig

IL-6 protein is a multifunctional cytokine that participates in immune, regenerative and metabolic processes by binding to IL6R and forming a complex with IL6ST/gp130. This activates the IL6 signaling pathway, initiating "classical signaling" via membrane-bound IL6R and IL6ST, "trans signaling" via binding of IL6 and soluble IL6R to IL6ST, and "cluster signaling" via the IL6:IL6R complex ". IL-6 Protein, Pig is the recombinant pig-derived IL-6 protein, expressed by E. coli , with tag free. The total length of IL-6 Protein, Pig is 184 a.a., with molecular weight of ~21.2 kDa.

IL-6 蛋白是一种多功能细胞因子，参与各种免疫、再生和代谢过程。它通过与 IL6R 结合而发挥作用，导致与信号亚基 IL6ST/gp130 形成复合物，从而激活细胞内 IL6 信号通路。这种相互作用可以触发不同类型的信号传导，包括通过膜结合的 IL6R 和 IL6ST 的“经典信号传导”、通过 IL6 和可溶性 IL6R 与 IL6ST 结合的“反式信号传导”，以及通过 IL6:IL6R 复合物的“簇信号传导”。发射细胞激活邻近接收细胞上的 IL6ST 受体。IL-6 对于急性期反应至关重要，在感染和组织损伤期间的宿主防御中发挥作用。然而，IL-6 的过量产生与疾病病理有关。它是由巨噬细胞和树突状细胞等骨髓细胞通过 Toll 样受体 (TLR) 响应病原体识别而合成的。在适应性免疫反应中，IL-6是B细胞分化为免疫球蛋白分泌细胞所必需的，并且在CD4(+) T细胞亚群的分化中发挥重要作用。它是滤泡辅助 T (Tfh) 细胞发育的重要因子，而 Tfh 细胞对于生发中心的形成以及在初始 CD4(+) T 细胞中诱导 Th17 谱系至关重要。此外，IL-6对于骨髓瘤细胞和浆母细胞的增殖和存活是必需的。

1.Measured by its binding ability in a functional ELISA. Immobilized sus scrofa (pig) IL6 at 2 μg/mL (100 μl/well) can bind Human IL-6R-His , the EC₅₀ of Human IL-6R-His is 250-750 ng/mL.
2. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED₅₀ for this effect is typically 1-5 ng/mL.

Pig

E. coli

Tag Free

P26893 (P29-M212)

399500  [NCBI]

Approximately 21.2 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.