IL-6R 蛋白, Canine (HEK293, His)

IL-6R alpha is a subunit alpha of IL-6 receptors, also shared by other interleukin receptors. IL-6R alpha acts as IL-6 agonist and involves in JAK/STAT, MAPK, and Akt signaling pathway^[1][2]. IL-6R alpha, Canine consists of 462 amino acids (M1-R462) and is produced in HEK293 cells with a C-Terminal His-tag.

IL-6 兼具炎症因子和抗炎因子的作用，通过激活一系列下游信号级联反应，包括 gp130（二聚体）、JAK/STAT、MAPK 和 Akt^[1]< super>[2]。
IL-6R α (IL-6Rα) 作为白细胞介素 6 受体的一部分，是一种 I 型跨膜糖蛋白，与 I 型跨膜信号形成复合物转导器糖蛋白 130 (CD130) 并以低亲和力调节 IL-6 的生物活性^[3]。
IL-6R α 有 2 种亚型，包括 mIL6R（较长的一种）或 sIL6R （较短的一个）：
mIL6R 是膜结合白细胞介素 6 受体，具有通过树突状细胞的“簇信号传导”将幼稚 CD4⁺ T 细胞驱动至 Th17 谱系的潜力细胞^[4]。
sIL-6R 是可溶性白细胞介素 6 受体亚基，从激活的 CD4⁺ 中的 IL-6R α (IL-6Rα) 裂解而来T细胞通过蛋白水解作用，并充当IL-6激动剂。sIL-6R 结合膜结合的 IL6R 和亚基 IL6ST，通过 IL-6 反式信号传导激活再生和抗炎信号，并促进 IL-6 的促炎特性。IL-6R α 的水解也称为胞外域脱落^[1]。
IL-6R α 参与调节细胞生长和分化，在免疫反应调节中发挥重要作用。急性期反应和造血^[5]。
然而，IL-6R α 在肝脏和免疫系统的一些细胞中表现出组织表达特异性，从而导致 IL6 信号传导受到限制^[6]。
值得注意的是，IL-6R α 失调与多种疾病的发病机制有关，例如多发性骨髓瘤、自身免疫性疾病和前列腺癌^[7][8]。

Measured by its binding ability in a functional ELISA.Immobilized canine IL6R-His at 10 μg/mL (100 μL/well) can bind human IL6, the EC₅₀ of human IL6 is 5-50 ng/mL.

Canine

HEK293

C-His

A0A8I3NUE0/XP_855105.1 (L20-P365)

612271  [NCBI]

LAPWGCPAPEVMSGVVTSLPGASVTLTCPGGEPEDNGTVQWLLQNLVDDSYLGSRAAVGRTLLLRSVQLSDSGNYSCYQDGRLAGTVRLLVDAPPEEPQLACFRKSPLSMVFCEWSPRHPPSPKTRATLLVRKFRGRPVGDSQEPCPYAQGPQKFSCQLAVPEGDNSLYVVSLCVTNSAGSRSSTPQTFEGYGILQPDPPVNITVTAVDRNPRWLRVSWQDPPSWNSYFYRLQFELRYRAERSKTFTMLMIKEFQHHCIIHDAWRGMRHVVQLRAREEFGHGSWSTWSQEAVGTPWTESRSPAAETELPHSTQAPTTNEDNKNNISKDIANATSLPVQDSSSAPLP

Approximately 39.8 kDa

Greater than 95% as determined by reducing SDS-PAGE

溶液.

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. Garbers C, et al. Inhibition of classic signaling is a novel function of soluble glycoprotein 130 (sgp130), which is controlled by the ratio of interleukin 6 and soluble interleukin 6 receptor. J Biol Chem. 2011 Dec 16;286(50):42959-70.  [Content Brief]

  [2]. Malemud, et al. Defective JAK-STAT Pathway Signaling Contributes to Autoimmune Diseases. Curr Pharmacol Rep 4, 358-366.

  [3]. Larsen JV, et al. SorLA in Interleukin-6 Signaling and Turnover. Mol Cell Biol. 2017 May 16;37(11):e00641-16.  [Content Brief]

  [4]. Kang S, et al. Targeting Interleukin-6 Signaling in Clinic. Immunity. 2019 Apr 16;50(4):1007-1023.  [Content Brief]

  [5]. Giannitrapani L, et al. Circulating IL-6 and sIL-6R in patients with hepatocellular carcinoma. Ann N Y Acad Sci. 2002 Jun;963:46-52.  [Content Brief]

  [6]. Arnold P, et al. Joint Reconstituted Signaling of the IL-6 Receptor via Extracellular Vesicles. Cells. 2020 May 24;9(5):1307.  [Content Brief]

  [7]. Mishra AK, et al. Metformin inhibits IL-6 signaling by decreasing IL-6R expression on multiple myeloma cells. Leukemia. 2019 Nov;33(11):2695-2709.  [Content Brief]

  [8]. Gruber CN, et al. Mapping Systemic Inflammation and Antibody Responses in Multisystem Inflammatory Syndrome in Children (MIS-C). Cell. 2020 Nov 12;183(4):982-995.e14.  [Content Brief]