IL-8/CXCL8 蛋白, Rhesus Macaque

Interleukin-8 (IL-8), also known as CXCL8 or NAP-1, is a pro-inflammatory CXC chemokine. IL-8 acts on human neutrophils via two receptors, CXCR1 and CXCR2. IL-8 has a conserved Glu-Leu-Arg (ELR) N-terminal motif, and is an agonist for CXCR1/CXCR2. IL-8 is produced by various cells including leukocytes, endothelial cells, and epithelial cells^[1][2][3]. IL-8/CXCL8 Protein, Rhesus Macaque is produced in E.coil, and consists of 79 amino acids (A23-P101).

IL-8 (CXCL8) 属于 ELR⁺ CXC 趋化因子家族。IL-8 最初产生为 99 个氨基酸的蛋白质，经过裂解形成活性 IL-8 同工型、非免疫细胞中的 77 个氨基酸肽或单核细胞和巨噬细胞中的 72 个氨基酸肽。编码IL-8的基因位于染色体4q13-q21上。IL-8 的二聚化形成了受体结合的结构基础。IL-8 由单核细胞、巨噬细胞、白细胞、内皮细胞和上皮细胞等多种细胞表达^[1][2][3]。
成熟的人IL-8/CXCL8与犬IL-8/CXCL8具有75%的氨基酸序列同一性。而人 IL-8 与恒河猴 IL-8 蛋白具有 94.95% 的氨基酸序列同一性。
IL-8 负责将中性粒细胞和粒细胞募集和激活至炎症部位。IL-8 在生理状态下几乎检测不到，但会被促炎细胞因子（例如 TNFα 和 IL-1β）快速诱导。IL-8的功能主要依赖于其与特定细胞表面GPCR、CXCR1和CXCR2的相互作用。此外，据报道，IL-8 可通过激活 PI3K/Akt 通路促进整合素 β3 上调和肝细胞癌细胞的侵袭。在牙源性病变中，IL-8已被证明在成釉细胞瘤上皮细胞和不可逆性牙髓炎中高表达。在类风湿性关节炎和其他炎症性关节疾病中，IL-8 可能导致中性粒细胞积聚，而中性粒细胞被认为是软骨降解酶的主要来源。IL-8 刺激细胞蛋白的 MAPK 和酪氨酸磷酸化。肿瘤细胞和成纤维细胞相互沟通，包括自分泌和旁分泌因子（包括 IL-8），导致 MMP2 和 MMP9 可降解细胞外基质 (ECM) 成分上调，从而触发肿瘤侵袭^[1]< support>[2]^[3][4]。
IL-8 通常已知可促进血管生成，但它也会激活基质金属蛋白酶（MMP）参与转移相关的组织重塑。IL-8 在脂多糖 (LPS) 刺激的单核细胞中被诱导，并显示出诱导中性粒细胞迁移。IL-8对肿瘤细胞的增殖、侵袭和迁移等生物学活性发挥多种作用。IL-8 还可增加雄激素非依赖性前列腺癌 (AIPC) 细胞系中 Akt 的表达。IL-8 通过中性粒细胞中的 PI3K 激活 MAPK 信号传导，并通过 EGFR 反式激活激活卵巢和肺癌细胞系中的 Ras-GTPase。大量实验数据表明，IL-8 和受体有助于消除病原体，但也可能对疾病相关过程产生显着影响，包括组织损伤、纤维化、血管生成和肿瘤发生^{[3] [5]}。

Recombinant human IL-8 (1, 3, and 1 μg/kg; single intravenous injection) injected into rhesus monkeys (2-3 years; 2.5-4.5 kg). IL-8 injection results in instant neutropenia that was due to pulmonary sequestration. Within 3 minutes after IL-8 injection, neutrophilia developed with counts up to 1-fold greater than baseline levels. The numbers of hematopoietic progenitor cells (HPCs) increased of blood at 3 minutes after injection of 1 μg/kg IL-8^[7].

Recombinant human IL-8/CXCL8 (1 ng/mL; for 24-96 h) stimulation leads to enhancement of invasion and suppression of late stage apoptosis in MG-63 cells. Moreover, secretions of MMPs by MG-63 cells are also increased upon stimulation. CXCL8 induces the elevations of phosphorylated PI3K and Akt, but not PKC or FAK^[6].

Rhesus Macaque

E. coli

Tag Free

P67813 (A23-P101)

613028  [NCBI]

Approximately 9.3 kDa

Greater than 95% as determined by reducing SDS-PAGE

<1 EU/μg, determined by LAL method.

• [1]. M Baggiolini, et al. Neutrophil-activating peptide-1/interleukin 8, a novel cytokine that activates neutrophils. J Clin Invest. 1989 Oct;84(4):1045-9.  [Content Brief]

  [2]. M Wolf, et al. Granulocyte chemotactic protein 2 acts via both IL-8 receptors, CXCR1 and CXCR2. Eur J Immunol. 1998 Jan;28(1):164-70.  [Content Brief]

  [3]. Qian Liu, et al. The CXCL8-CXCR1/2 pathways in cancer. Cytokine Growth Factor Rev. 2016 Oct;31:61-71.  [Content Brief]

  [4]. Jian-Feng Liu, et al. IL-8 Is Upregulated in the Tissue-Derived EVs of Odontogenic Keratocysts. Biomed Res Int. 2022 Jul 30;2022:9453270.  [Content Brief]

  [5]. Remo C Russo, et al. The CXCL8/IL-8 chemokine family and its receptors in inflammatory diseases. Expert Rev Clin Immunol. 2014 May;10(5):593-619.  [Content Brief]

  [6]. Hai Jiang, et al. CXCL8 promotes the invasion of human osteosarcoma cells by regulation of PI3K/Akt signaling pathway. APMIS. 2017 Sep;125(9):773-780.  [Content Brief]

  [7]. L Laterveer, et al. Rapid mobilization of hematopoietic progenitor cells in rhesus monkeys by a single intravenous injection of interleukin-8. Blood. 1996 Jan 15;87(2):781-8.  [Content Brief]