Insulin R/CD220 蛋白, Human (sf9, His-GST)

The insulin R/CD220 protein is a receptor tyrosine kinase that coordinates insulin action by phosphorylating substrates such as IRS and SHC upon insulin binding. Phosphorylated IRS activates PI3K-AKT/PKB to exert metabolic effects, and activates Ras-MAPK to promote growth and differentiation. Insulin R/CD220 Protein, Human (sf9, His-GST) is the recombinant human-derived Insulin R/CD220 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.

胰岛素 R/CD220 蛋白是一种受体酪氨酸激酶，通过胰岛素结合后多种细胞内底物的磷酸化来协调胰岛素的多种作用。这些底物包括胰岛素受体底物（IRS1、2、3、4）、SHC、GAB1、CBL 和其他信号传导中间体。磷酸化 IRS 蛋白激活两条主要信号传导途径：PI3K-AKT/PKB 途径（对于胰岛素的代谢作用至关重要）和 Ras-MAPK 途径（与 PI3K 协作调节细胞生长和分化）。PI3K-AKT/PKB 途径触发葡萄糖转运蛋白 SLC2A4/GLUT4 易位至细胞膜，促进葡萄糖转运。激活的下游效应子AKT/PKB通过磷酸化BAD诱导抗凋亡作用，调节代谢酶的表达，并调节mTORC1信号通路，整合胰岛素信号促进细胞生长和代谢。Ras/RAF/MAP2K/MAPK 通路介导胰岛素诱导的细胞生长、存活和细胞分化。除胰岛素外，该受体还可以结合胰岛素样生长因子（IGFI 和 IGFII）。由 IGF1R 和 INSR 亚型组成的混合受体对 IGF 和胰岛素表现出不同的亲和力，影响它们的激活模式。此外，在脂肪细胞中，受体抑制脂肪分解。

The specific activity was determined to be >45 nmol/min/mg using Poly (Ala,Glu,Lys,Tyr) 6:2:5:1 as substrate.

Human

Sf9 insect cells

N-His;N-GST

P06213-1/NP_000199.2 (G989-S1382)

3643  [NCBI]

Approximately 70 kDa

Greater than 90% as determined by reducing SDS-PAGE

溶液.

Supplied as a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, pH 7.4, 20% gly, 0.3 mM DTT

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.