CD47 蛋白, Cynomolgus/Rhesus Macaque (HEK293, Fc)

CD47 is a leukocyte surface antigen, and high expression of CD47 helps tumors escape. CD47 inhibition leads to the activation of CD103+ dendritic cells, which leads to the recruitment and activation of natural killer cells and inhibits cancer development. CD47 Protein, Rhesus Macaque (HEK293, Fc) is the recombinant Rhesus Macaque-derived CD47 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of CD47 Protein, Rhesus Macaque (HEK293, Fc) is 121 a.a., with molecular weight of 60-90 kDa.

CD47，是白细胞表面抗原 (Leukocyte surface antigen) 和血小板反应蛋白 C 端细胞结合域的受体。当细胞粘附到细胞外基质时，CD47 控制细胞内钙浓度的增加，可能在膜运输和信号转导中发挥作用。CD47 基因具有广泛的组织分布，并且在 Rh 红细胞上的表达低。高表达水平的 CD47 帮助癌细胞逃避免疫系统。如研究发现，CD47 的高表达与 HCC 患者预后较差相关。CD47 抑制，可以增强 CD103+ DCs 摄取肿瘤 DNA 的能力，从而刺激 cGAS-STING 通路，促进肝脏自然杀伤细胞在癌细胞中的浸润和活化。CD47 还结合包括血小板反应蛋白-1 (TSP-1)、信号调节蛋白 α (SIRPα)、整合素和带有 SH2 结构域的蛋白酪氨酸磷酸酶底物-1 (SHPS-1)在内的配体。而调节巨噬细胞的细胞吞噬作用、中性粒细胞的迁移以及树突状细胞、T 细胞和 B 细胞的激活。一些 CD47 抗体可能抑制 CD47-SIRPα 轴来增强癌细胞的吞噬作用。

1.10 μg/mL (100 μL/well) of immobilized recombinant Rhesus Macaque Integrin-associated protein/CD47-Fc can bind Anti-Human CD47 mAb-IgG4Fc with an ED₅₀ value of 76.1 ng/mL.
2.Measured by its binding ability in a functional ELISA. Immobilized Human SIRP alpha at 2μg/mL (100 μL/well) can bind Cynomolgus CD47. The ED₅₀ for this effect is 0.19 μg/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized Human SIRP alpha at 2μg/mL (100 μL/well) can bind Cynomolgus CD47. The ED₅₀ for this effect is 0.19 μg/mL.

Rhesus Macaque; Cynomolgus

HEK293

C-hFc

F7A802/NP_001253446 (Q19-S139)

704980  [NCBI]/102139846

QLLFNKTKSVEFTFCNDTVVIPCFVTNMEAQNTTEVYVKWKFKGRDIYTFDGALNKSTAPANFSSAKIEVSQLLKGDASLKMDKSDAVSHTGNYTCEVTELTREGETIIELKYRVVSWFS

Approximately 60-90 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 100 mM Glycine, pH 7.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.