ISCU 蛋白, Human (Sf9, His)

ISCU is an important mitochondrial scaffolding protein in the ISC assembly complex and forms the structural basis of [2Fe-2S] cluster assembly. ISCU relies on FXN to centrally receive persulfide during de novo synthesis initiated by the cysteine desulfurase complex (NFS1:LYRM4:NDUFAB1). ISCU Protein, Human (Sf9, His) is the recombinant human-derived ISCU protein, expressed by Sf9 insect cells , with N-6*His labeled tag.

ISCU 是核心铁硫簇 (ISC) 组装复合物中的关键线粒体支架蛋白，是 [2Fe-2S] 簇组装的结构基础。在由半胱氨酸脱硫酶复合物 (NFS1:LYRM4:NDUFAB1) 启动的 [2Fe-2S] 簇从头合成的复杂过程中，ISCU 通过以 FXN 依赖性方式接收过硫化物而发挥核心作用。FDX2 促进了该复合物的稳定，为有效的 [2Fe-2S] 簇组装提供了还原当量。随后，ISCU 充当关键的中介，将组装的 [2Fe-2S] 簇转移到伴侣蛋白，包括 HSCB、HSPA9 和 GLRX5。值得注意的是，ISCU 表现出动态构象状态，在结构化 (S) 和无序 (D) 形式之间交替。它对 NFS1 脱硫酶活性的锌依赖性调节以及对 FXN 和半胱氨酸脱硫酶复合物之间相互作用的影响进一步强调了其多方面的作用。此外，作为细胞质支架蛋白，ISCU 有助于细胞质 ISC 组装复合物的结构框架，参与 Fe-S 簇的组装，并可能有助于细胞质铁硫蛋白的生物发生。

Human

Sf9 insect cells

N-6*His

Q9H1K1-1 (Y35-K167)

23479  [NCBI]

YHKKVVDHYENPRNVGSLDKTSKNVGTGLVGAPACGDVMKLQIQVDEKGKIVDARFKTFGCGSAIASSSLATEWVKGKTVEEALTIKNTDIAKELCLPPVKLHCSMLAEDAIKAALADYKLKQEPKKGEAEKK

Approximately 17 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm solution of PBS, pH 7.4, 10% Glycerol, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.