1. 重组蛋白
  2. ISG15/UCRP 蛋白, Human (C-6*His)

ISG15/UCRP 蛋白, Human (C-6*His)

目录号: HY-P70149A
COA 产品使用指南

ISG15/UCRP 蛋白, Human (C-6*His) 是重组的 ISG15/UCRP 蛋白,由 E. coli 表达,带有 C-6*His 标签。ISG15/UCRP 蛋白, Human (C-6*His)

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规格 价格 是否有货 数量
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5 μg ¥300 In-stock
10 μg ¥496 In-stock
20 μg ¥780 In-stock
50 μg   询价  
100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

ISG15/UCRP Protein, Human (C-6*His) is the recombinant human-derived ISG15/UCRP, expressed by E. coli , with C-6*His labeled tag. ,

生物活性

Measured in a cell proliferation assay using PANC-1 cells. The ED50 for this effect is typically 16.47 ng/mL.

种属

Human

表达系统

E. coli

标签

C-6*His

蛋白编号

AAH09507.1 (G2-G157)

基因 ID

9636

蛋白结构
N-term
ISG15 (G2-G157)
Accession # AAH09507.1
6*His
C-term
同用名
rHuISG15/UCRP, His; Ubiquitin-Like Protein ISG15; Interferon-Induced 15 kDa Protein; Interferon-Induced 17 kDa Protein; IP17; Ubiquitin Cross-Reactive Protein; hUCRP; ISG15; G1P2; UCRP
氨基酸序列

GWDLTVKMLAGNEFQVSLSSSMSVSELKAQITQKIGVHAFQQRLAVHPSGVALQDRVPLASQGLGPGSTVLLVVDKCDEPLNILVRNNKGRSSTYEVRLTQTVAHLKQQVSGLEGVQDDLFWLTFEGKPLEDQLPLGEYGLKPLSTVFMNLRLRGG

分子量

Approximately 17.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末

组分

Lyophilized from a 0.22 μm filtered solution of 50 mM Tris-HCL, pH 8.0, 5% trehalose, 5% mannitol and 0.01% Tween80.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
ISG15/UCRP Protein, Human (C-6*His)
目录号:
HY-P70149A
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