ITCH/AIP4 蛋白, Human (solution)

The ITCH/AIP4 protein, a member of the Nedd4 family of ubiquitin ligases, transfers ubiquitin to protein substrates for lysosomal degradation. It regulates cell differentiation and immune responses and is associated with autoimmune disease. Alternative splicing produces multiple isoforms. ITCH/AIP4 is expressed ubiquitously, with notable levels in the testis, esophagus, and 25 other tissues. ITCH/AIP4 Protein, Human (solution) is the recombinant human-derived ITCH/AIP4 protein, expressed by E. coli , with tag free. and with residual Gly-Pro in N-terminal (not related to functional changes).

ITCH/AIP4 蛋白是 HECT 结构域 E3 泛素连接酶 Nedd4 家族的成员，参与泛素从 E2 泛素结合酶到蛋白质底物的转移，导致其在溶酶体中降解。这种蛋白质具有多种功能，包括调节红细胞和淋巴细胞分化以及免疫反应。该基因的突变与综合征性多系统自身免疫性疾病有关。此外，选择性剪接会导致该蛋白质产生多种亚型。ITCH/AIP4 蛋白表现出无处不在的表达，在睾丸、食道和 25 种其他组织等组织中表达水平显着。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

Tag Free

NP_113671.3 (N-G&P, R485-E862)

83737  [NCBI]

Approximately 40 kDa

Greater than 95% as determined by reducing SDS-PAGE

溶液.

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 200 mM NaCl, 10% Glycerol, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.