1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. ITPase 蛋白, Human (His)

ITPase 蛋白, Human (His)

目录号: HY-P70904
产品使用指南

ITPase蛋白是核苷酸代谢中必需的焦磷酸酶,可以将ITP、dITP、dHAPTP和XTP等非经典嘌呤核苷酸水解成各自的单磷酸衍生物。它缺乏脱氧和核糖形式之间的区别。ITPase 蛋白, Human (His) 是重组的 ITP 酶蛋白,由 E. coli 表达,带有 C-6*His 标签。

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描述

ITPase protein is a pyrophosphatase that is essential in nucleotide metabolism and can hydrolyze non-classical purine nucleotides such as ITP, dITP, dHAPTP and XTP into their respective monophosphate derivatives. It lacks distinction between deoxy and ribose forms. ITPase Protein, Human (His) is the recombinant human-derived ITPase protein, expressed by E. coli , with C-6*His labeled tag.

研究背景

ITPase 是一种焦磷酸酶,通过水解非经典嘌呤核苷酸(包括肌苷三磷酸 (ITP)、脱氧肌苷三磷酸 (dITP)、2'-脱氧-N-6-羟氨基嘌呤三磷酸 (dHAPTP) 和黄苷)在核苷酸代谢中发挥关键作用5'-三磷酸(XTP)转化为各自的单磷酸衍生物。该酶表现出脱氧糖和核糖形式之间缺乏区别。据推测,ITPase 的主要功能是从 RNA 和 DNA 前体库中排除非规范嘌呤。通过阻止这些嘌呤掺入 RNA 和 DNA,ITPase 保护遗传物质的完整性,有助于维持基因组稳定性并避免潜在的染色体损伤。

种属

Human

表达系统

E. coli

标签

C-6*His

蛋白编号

Q9BY32-1 (A2-A194)

基因 ID
蛋白结构
N-term
ITPase (A2-A194)
Accession # Q9BY32-1
6*His
C-term
同用名
Inosine Triphosphate Pyrophosphatase; ITPase; Inosine Triphosphatase; Non-Canonical Purine NTP Pyrophosphatase; Non-Standard Purine NTP Pyrophosphatase; Nucleoside-Triphosphate Diphosphatase; Nucleoside-Triphosphate Pyrophosphatase; NTPase; Putative Oncogene Prot
氨基酸序列

AASLVGKKIVFVTGNAKKLEEVVQILGDKFPCTLVAQKIDLPEYQGEPDEISIQKCQEAVRQVQGPVLVEDTCLCFNALGGLPGPYIKWFLEKLKPEGLHQLLAGFEDKSAYALCTFALSTGDPSQPVRLFRGRTSGRIVAPRGCQDFGWDPCFQPDGYEQTYAEMPKAEKNAVSHRFRALLELQEYFGSLAA

分子量

Approximately 21.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

ITPase Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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