1. 重组蛋白
  2. Enzymes & Regulators
  3. Protein Tyrosine Kinases
  4. JAK2
  5. Janus kinase 2/JAK2 蛋白, Human (381aa, His)

Janus kinase 2/JAK2 蛋白, Human (381aa, His)

目录号: HY-P701102
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Janus kinase 2/JAK2 蛋白, Human 是一种在大肠杆菌中表达的,N 末端有 His 标签的重组人 JAK2。JAK2 是一种非受体酪氨酸激酶。

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20 μg ¥1650 询问价格 & 货期
50 μg ¥3100 询问价格 & 货期

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描述

Janus kinase 2/JAK2 Protein, Human is a recombinant human JAK2 expressed in E. coli with a His tag at the N-terminus. JAK2 is a non-receptor tyrosine kinase[1][2].

研究背景

JAK2 是非受体蛋白酪氨酸激酶 Janus 激酶 (JAK) 家族的成员,该家族包括 JAK1-3 和 TYK2。JAK 作为细胞因子受体的细胞质信号传导成分,通过细胞因子介导的转磷酸化被激活,从而导致受体磷酸化以及信号转导子和转录激活子 (STAT) 蛋白的募集和磷酸化。JAK 是非受体蛋白酪氨酸激酶,作为多种细胞因子受体的催化信号传导成分,包括白细胞介素、干扰素、生长激素、促红细胞生成素和瘦素的受体[1][2]

种属

Human

表达系统

E. coli

标签

N-6*His

蛋白编号

O60674-1 (H752-G1132)

基因 ID
蛋白结构
N-term
6*His
JAK2 (H752-G1132)
Accession # O60674-1
C-term
同用名
JAK 2; JAK-2; JAK2; JAK2_HUMAN; Janus Activating Kinase 2; Janus kinase 2 (a protein tyrosine kinase); Janus kinase 2; JTK 10; JTK10; kinase Jak2; OTTHUMP00000043260; THCYT3; Tyrosine protein kinase JAK2; Tyrosine-protein kinase JAK2
氨基酸序列

KPLSALDSQRKLQFYEDRHQLPAPKWAELANLINNCMDYEPDFRPSFRAIIRDLNSLFTPDYELLTENDMLPNMRIGALGFSGAFEDRDPTQFEERHLKFLQQLGKGNFGSVEMCRYDPLQDNTGEVVAVKKLQHSTEEHLRDFEREIEILKSLQHDNIVKYKGVCYSAGRRNLKLIMEYLPYGSLRDYLQKHKERIDHIKLLQYTSQICKGMEYLGTKRYIHRDLATRNILVENENRVKIGDFGLTKVLPQDKEYYKVKEPGESPIFWYAPESLTESKFSVASDVWSFGVVLYELFTYIEKSKSPPAEFMRMIGNDKQGQMIVFHLIELLKNNGRLPRPDGCPDEIYMIMTECWNNNVNQRPSFRDLALRVDQIRDNMAG

分子量

Approximately 48.6 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

Janus kinase 2/JAK2 Protein, Human (381aa, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Janus kinase 2/JAK2 Protein, Human (381aa, His)
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HY-P701102
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