1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. JOSD1 蛋白, Human

JOSD1 蛋白, Human

目录号: HY-P701474
产品使用指南

JOSD1 蛋白是泛素动力学中的多功能参与者,并通过在体外裂解单泛素化探针来表现出去泛素酶活性。当泛素化时,JOSD1 可以在体外裂解“Lys-63”连接和“Lys-48”连接的多聚泛素链,表明其去泛素化的潜力。JOSD1 蛋白, Human 是重组的 JOSD1 蛋白,由 E. coli 表达,不带标签。JOSD1 蛋白, Human 全长 201 个氨基酸。

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描述

The JOSD1 protein is a versatile player in ubiquitin dynamics and exhibits deubiquitinase activity by cleaving monoubiquitinated probes in vitro. When ubiquitinated, JOSD1 can cleave “Lys-63”-linked and “Lys-48”-linked polyubiquitin chains in vitro, suggesting its potential for deubiquitination. JOSD1 Protein, Human is the recombinant human-derived JOSD1 protein, expressed by E. coli , with tag free. The total length of JOSD1 Protein, Human is 201 a.a., .

研究背景

JOSD1 蛋白是泛素动力学中的多功能参与者,通过在体外切割单泛素化探针来表现出去泛素酶活性。此外,当自身泛素化时,JOSD1 可以在体外裂解“Lys-63”连接和“Lys-48”连接的多聚泛素链,这表明其具有作为去泛素化酶的潜在作用。除了其催化功能外,JOSD1 还参与细胞过程,积极影响巨胞饮作用,同时抑制网格蛋白和小凹介导的内吞作用。值得注意的是,JOSD1 似乎独立于其酶活性而对膜动力学和细胞运动做出贡献,突出了其在协调细胞反应和稳态中的多方面作用。

种属

Human

表达系统

E. coli

标签

Tag Free

蛋白编号

Q15040 (S2-V202)

基因 ID

9929

蛋白结构
N-term
JOSD1 (S2-V202)
Accession # Q15040
C-term
同用名
JOSD1; Josephin-1; Josephin domain-containing protein 1
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

JOSD1 Protein, Human 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
JOSD1 Protein, Human
目录号:
HY-P701474
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