1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. Kallikrein-7 蛋白, Mouse (HEK293, His)

Kallikrein-7 蛋白, Mouse (HEK293, His)

目录号: HY-P70883
产品使用指南

Kallikrein-7 是皮肤完整性的重要贡献者,可能会催化细胞间粘附结构的降解,而细胞间粘附结构对于连续细胞脱落至关重要。Kallikrein-7 对胰岛素 A 链和 B 链表现出裂解活性,对 P1 位置的芳香族残基具有特异性,表明底物识别具有多功能性。Kallikrein-7 蛋白, Mouse (HEK293, His) 是重组的 Kallikrein-7 蛋白,由 HEK293 表达,带有 C-6*His 标签。

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描述

Kallikrein-7 is an important contributor to skin integrity and may catalyze the degradation of intercellular adhesive structures that are critical for continuous cell shedding.Kallikrein-7 exhibits cleavage activity against insulin A and B chains, with specificity for aromatic residues at position P1 indicating versatility in substrate recognition.Kallikrein-7 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Kallikrein-7 protein, expressed by HEK293 , with C-6*His labeled tag.

研究背景

Kallikrein-7 蛋白是维持皮肤完整性的重要贡献者,有可能催化角质层内细胞间粘合结构的降解。这种活动可能对于皮肤表面细胞的持续脱落至关重要。由于对 P1 位上具有芳香侧链的氨基酸残基具有特异性,Kallikrein-7 对胰岛素 A 和 B 链的特定残基表现出裂解活性,表明其在底物识别方面具有多功能性。此外,该蛋白质可能在炎症细胞因子前体的激活中发挥作用,这意味着可能参与炎症过程的调节。Kallikrein-7 的多方面功能强调了其在皮肤生理学中的重要性及其对结构完整性和炎症反应的潜在贡献,值得进一步探索其特定的分子机制。

种属

Mouse

表达系统

HEK293

标签

C-6*His

蛋白编号

Q91VE3 (Q22-R249)

基因 ID
蛋白结构
N-term
Kallikrein-7 (Q22-R249)
Accession # Q91VE3
6*His
C-term
同用名
Kallikrein-7; Klk7; Serine protease 6; Stratum corneum chymotryptic enzyme; Thymopsin; kallikrein-related peptidase 7; PRSS6; SCCEkallikrein-7
氨基酸序列

QGERIIDGYKCKEGSHPWQVALLKGNQLHCGGVLVDKYWVLTAAHCKMGQYQVQLGSDKIGDQSAQKIKATKSFRHPGYSTKTHVNDIMLVRLDEPVKMSSKVEAVQLPEHCEPPGTSCTVSGWGTTTSPDVTFPSDLMCSDVKLISSRECKKVYKDLLGKTMLCAGIPDSKTNTCNGDSGGPLVCNDTLQGLVSWGTYPCGQPNDPGVYTQVCKYKRWVMETMKTHR

分子量

28-35 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

Kallikrein-7 Protein, Mouse (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Kallikrein-7 Protein, Mouse (HEK293, His)
目录号:
HY-P70883
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