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  3. Kilon/NEGR1 蛋白, Mouse (HEK293, His)

Kilon/NEGR1 蛋白, Mouse (HEK293, His)

目录号: HY-P75934
COA 产品使用指南

Kilon/NEGR1 蛋白与细胞粘附有关,表明其在重要的细胞相互作用中发挥作用。它作为跨神经元生长促进因子的潜在功能突出了其在促进哺乳动物大脑再生轴突萌芽中的动态作用。Kilon/NEGR1 蛋白, Mouse (HEK293, His) 是重组的 Kilon/NEGR1 蛋白,由 HEK293 表达,带有 C-His 标签。Kilon/NEGR1 蛋白, Mouse (HEK293, His) 全长 287 个氨基酸,分子量约为 ~47 kDa。

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描述

The Kilon/NEGR1 protein has been implicated in cell adhesion, suggesting a role in important cellular interactions. Its potential function as a transneuronal growth-promoting factor highlights its dynamic role in promoting regenerative axonal sprouting in the mammalian brain. Kilon/NEGR1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Kilon/NEGR1 protein, expressed by HEK293 , with C-His labeled tag. The total length of Kilon/NEGR1 Protein, Mouse (HEK293, His) is 287 a.a., with molecular weight of ~47 kDa.

研究背景

Kilon/NEGR1 蛋白作为细胞粘附的潜在参与者而出现,表明其在介导细胞过程的关键相互作用中发挥着作用。此外,其作为跨神经生长促进因子的潜在功能突出了其在促进哺乳动物大脑再生轴突萌芽中的动态作用。Kilon/NEGR1 参与再生过程意味着它在促进轴突生长方面具有重要意义,特别是在大脑内神经再生的背景下。探索 Kilon/NEGR1 促进细胞粘附和促进再生轴突萌发的具体机制可能会为其在神经发育和再生中的作用提供有价值的见解。对 Kilon/NEGR1 功能的进一步研究可以加深我们对其对哺乳动物大脑神经元可塑性和再生过程的潜在影响的理解。

种属

Mouse

表达系统

HEK293

标签

C-His

蛋白编号

Q80Z24/NP_001034183.1 (V32-G318)

基因 ID
蛋白结构
N-term
Kilon (V32-G318)
Accession # Q80Z24/NP_001034183.1
His
C-term
同用名
Neuronal growth regulator 1; Kindred of IgLON; Kilon; Neurotractin; Kiaa3001; Ntra
氨基酸序列

VDFPWAAVDNMLVRKGDTAVLRCYLEDGASKGAWLNRSSIIFAGGDKWSVDPRVSISTLNKRDYSLQIQNVDVTDDGPYTCSVQTQHTPRTMQVHLTVQVPPKIYDISNDMTINEGTNVTLTCLATGKPEPVISWRHISPSAKPFENGQYLDIYGITRDQAGEYECSAENDVSFPDVKKVRVIVNFAPTIQEIKSGTVTPGRSGLIRCEGAGVPPPAFEWYKGEKRLFNGQQGIIIQNFSTRSILTVTNVTQEHFGNYTCVAANKLGTTNASLPLNPPSTAQYGITG

分子量

Approximately 43-55 kDa due to the glycosylation

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Kilon/NEGR1 Protein, Mouse (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Kilon/NEGR1 Protein, Mouse (HEK293, His)
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