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  3. Kirrel1/NEPH1 蛋白, Human (HEK293, Fc)

Kirrel1/NEPH1 蛋白, Human (HEK293, Fc)

目录号: HY-P76467
产品使用指南

Kirrel1/NEPH1 蛋白对于肾脏肾小球滤过屏障的正常功能至关重要,通过指状足突和间隙间隔细胞-细胞连接维持稳定的足细胞结构。作为信号分子,它需要TEC激酶才能完全激活转录因子AP-1。Kirrel1/NEPH1 蛋白, Human (HEK293, Fc) 是重组的 Kirrel1/NEPH1 蛋白,由 HEK293 表达,带有 C-hFc 标签。

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描述

The Kirrel1/NEPH1 protein is essential for the normal function of the glomerular filtration barrier in the kidney, maintaining stable podocyte structure through finger-like foot processes and gap septal cell-cell junctions. As a signaling molecule, it requires TEC kinase to fully activate the transcription factor AP-1. Kirrel1/NEPH1 Protein, Human (HEK293, Fc) is the recombinant human-derived Kirrel1/NEPH1 protein, expressed by HEK293 , with C-hFc labeled tag.

研究背景

Kirrel1/NEPH1 蛋白对于肾脏肾小球滤过屏障的正常功能至关重要。它通过促进由称为狭缝隔膜的特殊细胞与细胞连接点连接的叉指足突,在维持稳定的足细胞结构方面发挥着至关重要的作用。该蛋白充当信号分子,需要 TEC 激酶的存在才能完全激活转录因子 AP-1。Kirrel1/NEPH1 与 TJP1/ZO-1 和 NPHS2/podocin 相互作用,其与 NPHS1/nephrin 的相互作用依赖于 Kirrel1/NEPH1 糖基化。它通过其 Ig 样结构域形成同二聚体,并且当酪氨酸磷酸化时,它与 GRB2 相互作用。

种属

Human

表达系统

HEK293

标签

C-hFc

蛋白编号

Q96J84-1 (Q17-L493)

基因 ID
蛋白结构
N-term
Kirrel1/NEPH1 (Q17-L493)
Accession # Q96J84-1
hFc
C-term
同用名
Kin of IRRE-like protein 1; Kin of irregular chiasm-like protein 1; Nephrin-like protein 1; Kirrel1; Neph1
分子量

Approximately 96.1 kDa.

纯度

Greater than 95% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

Kirrel1/NEPH1 Protein, Human (HEK293, Fc) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Kirrel1/NEPH1 Protein, Human (HEK293, Fc)
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HY-P76467
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