kshB 蛋白, Mycobacterium tuberculosis (His)

The kshB protein significantly degrades cholesterol by catalyzing the introduction of the 9a-hydroxyl moiety into 1,4-androstadiene-3,17-dione (ADD), resulting in the 9OHADD intermediate. It is spontaneously converted to HSA through the cleavage of the B ring and aromatization of the A ring. kshB Protein, Mycobacterium tuberculosis (His) is the recombinant kshB protein, expressed by E. coli , with N-6*His labeled tag. The total length of kshB Protein, Mycobacterium tuberculosis (His) is 358 a.a., .

kshB 蛋白在胆固醇降解中起着至关重要的作用，催化将 9a-羟基部分引入 1,4-雄甾二烯-3,17-二酮 (ADD) 中，生成 9α-羟基-1,4-雄甾二烯- 3,17-二酮(9OHADD)中间体。该中间体通过环 B 的间位裂解自发转化为 3-羟基-9,10-seconandrost-1,3,5(10)-triene-9,17-dione (HSA)，同时环的芳构化A. 此外，kshB 在底物利用方面表现出多功能性，因为它还可以作用于 4-androstene-3,17-dione (AD)、3-oxo-23,24-bisnorcholesta-4-en-22-oate (4- BNC)、3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate (1,4-BNC)、3-oxo-23,24-bisnorcholesta-4-en-22-oyl-辅酶 A硫酯 (4-BNC-CoA) 和 3-oxo-23,24-bisnorcholesta-1,4-dien-22-oyl-coenzyme A 硫酯 (1,4-BNC-CoA) 作为底物。这凸显了该蛋白质在胆固醇分解代谢中的关键作用及其参与多种代谢途径的能力。

Others

E. coli

N-6*His

P9WJ93 (M1-E358)

887315

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.