Lactoferrin/LTF 蛋白, Human (691a.a, HEK293, His)

Lactoferrin (LTF) is a major iron-binding protein in exocrine fluid and exhibits multiple antibacterial effects by chelating iron and disrupting bacterial membranes. It prevents biofilm formation, affects bone metabolism, and affects adenovirus and papillomavirus infections. Lactoferrin/LTF Protein, Human (691a.a, HEK293, His) is the recombinant human-derived Lactoferrin/LTF protein, expressed by HEK293 , with C-6*His labeled tag.

乳铁蛋白 (LTF) 是一种主要的铁结合多功能蛋白，存在于母乳和粘膜分泌物等外分泌液中，具有多种生物活性。LTF 以其抗菌特性而闻名，它通过隔离游离铁并从细菌外膜释放脂多糖来阻碍微生物生长，从而发挥抑菌和杀菌作用。此外，它在防止铜绿假单胞菌感染中细菌生物膜的形成方面发挥作用，并对白色念珠菌表现出较弱的抗真菌活性。除了抗菌功能外，LTF还通过对成骨细胞发挥合成代谢、分化和抗凋亡作用来影响骨代谢，同时还抑制破骨细胞生成。它通过促进 C 种腺病毒与上皮细胞的结合来促进腺病毒感染，并抑制乳头瘤病毒感染。LTF 刺激 TLR4 信号传导通路，导致 NF-κ-B 激活和促炎细胞因子产生，同时干扰脂多糖刺激的 TLR4 信号传导。此外，它对中性粒细胞迁移具有抑制作用，并促进 VEGFA 介导的内皮细胞迁移和增殖。LTF 的多功能性延伸至其结合能力，包括与肝素、硫酸软骨素、肺炎球菌表面蛋白 A、溶菌酶和 DNA 的相互作用。

Human

HEK293

C-6*His

AAH15822.1 (G20-K711)

4057  [NCBI]

GSRRRSVQWCAVSQPEATKCFQWQRNMRKVRGPPVSCIKRDSPIQCIQAIAENRADAVTLDGGFIYEAGLAPYKLRPVAAEVYGTERQPRTHYYAVAVVKKGGSFQLNELQGLKSCHTGLRRTAGWNVPIGTLRPFLNWTGPPEPIEAAVARFFSASCVPGADKGQFPNLCRLCAGTGENKCAFSSQEPYFSYSGAFKCLRDGAGDVAFIRESTVFEDLSDEAERDEYELLCPDNTRKPVDKFKDCHLARVPSHAVVARSVNGKEDAIWNLLRQAQEKFGKDKSPKFQLFGSPSGQKDLLFKDSAIGFSRVPPRIDSGLYLGSGYFTAIQNLRKSEEEVAARRARVVWCAVGEQELRKCNQWSGLSEGSVTCSSASTTEDCIALVLKGEADAMSLDGGYVYTAGKCGLVPVLAENYKSQQSSDPDPNCVDRPVEGYLTVAVVRRSDTSLTWNSVKGKKSCHTAVDRTAGWNIPMGLLFNQTGSCKFDEYFSQSCAPGSDPRSNLCALCIGDEQGENKCEPNSNERYYGYTGAFRCLAENAGDVAFVKDVTVLQNTDGNNNEAWAKDLKLADFALLCLDGKRKPVTEARSCHLAMAPNHAVVSRMDKVERLKQVLLHQQAKFGRNGSDCPDKFCLFQSETKNLLFNDNTECLARLHGKTTYEKYLGPQYVAGITNLKKCSTSPLLEACEFLRK

Approximately 83 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.