LAMP1/CD107a 蛋白, Human (HEK293, His)

LAMP1/CD107a Protein, Human (HEK293, His) is a recombinant human CD107a expressed in HEK 293 cells with a His tag at the N-terminus. LAMP1/CD107a Protein can be used as a functional marker for the identification of natural killer cell activity^[1][2].

溶酶体相关膜蛋白 1（LAMP1 或 CD107a）是一种高度糖基化的溶酶体和内体膜蛋白，大小为 120 kDa。LAMP 和 LIMP 紧密堆积，占晚期内体和溶酶体总膜蛋白的 50% 以上。LAMP1 被描述为刺激后 CD8⁺ T 细胞脱粒的标志物。LAMP1 可作为识别自然杀伤细胞活性的功能标记^[1][2]。

Human

HEK293

C-6*His

P11279-1 (A29-M382)

3916  [NCBI]

AMFMVKNGNGTACIMANFSAAFSVNYDTKSGPKNMTFDLPSDATVVLNRSSCGKENTSDPSLVIAFGRGHTLTLNFTRNATRYSVQLMSFVYNLSDTHLFPNASSKEIKTVESITDIRADIDKKYRCVSGTQVHMNNVTVTLHDATIQAYLSNSSFSRGETRCEQDRPSPTTAPPAPPSPSPSPVPKSPSVDKYNVSGTNGTCLLASMGLQLNLTYERKDNTTVTRLLNINPNKTSASGSCGAHLVTLELHSEGTTVLLFQFGMNASSSRFFLQGIQLNTILPDARDPAFKAANGSLRALQATVGNSYKCNAEEHVRVTKAFSVNIFKVWVQAFKVEGGQFGSVEECLLDENSMHHHHHH

60-120 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against 20 mM PB, 150 mM NaCl, pH 7.2.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Alter G, et, al. CD107a as a functional marker for the identification of natural killer cell activity. J Immunol Methods. 2004 Nov;294(1-2):15-22.  [Content Brief]

  [2]. Eskelinen EL, et, al. At the acidic edge: emerging functions for lysosomal membrane proteins. Trends Cell Biol. 2003 Mar;13(3):137-45.  [Content Brief]