LCAT 蛋白, Human (HEK293, His)

LCAT protein is synthesized in the liver and plays a vital role in the metabolism of plasma lipoproteins by converting cholesterol and phosphatidylcholine in HDL and LDL. LCAT Protein, Human (HEK293, His) is the recombinant human-derived LCAT protein, expressed by HEK293 , with C-6*His labeled tag. The total length of LCAT Protein, Human (HEK293, His) is 416 a.a., with molecular weight of ~71 kDa.

LCAT 是血浆脂蛋白细胞外代谢的关键酶，主要在肝脏中合成并分泌到血浆中。在这一关键作用中，LCAT 将高密度脂蛋白和低密度脂蛋白（HDL 和 LDL）表面的胆固醇和磷脂酰胆碱（卵磷脂）转化为胆固醇酯和溶血磷脂酰胆碱。产生的胆固醇酯被转运回肝脏。LCAT 对血浆 16:0-18:2 或 18:O-18:2 磷脂酰胆碱表现出偏好。除了其肝脏功能外，LCAT 还由初级星形胶质细胞在大脑中产生，在胶质细胞分泌的新生含 APOE 脂蛋白上酯化游离胆固醇，影响脑脊液 (CSF) APOE- 和 APOA1 水平。LCAT 与 APOE 和胆固醇转运蛋白 ABCA1 合作，在神经胶质源性新生脂蛋白的成熟中发挥着关键作用。此外，LCAT 对于将高密度脂蛋白颗粒重塑为球形至关重要，并催化血小板活化因子 (PAF) 水解为溶血 PAF，以及 (24S)-羟基胆固醇 (24(S)OH) 的酯化-C)，称为脑甾醇，产生 24(S)OH-C 单酯。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

HEK293

C-6*His

P04180 (F25-E440)

3931  [NCBI]

FWLLNVLFPPHTTPKAELSNHTRPVILVPGCLGNQLEAKLDKPDVVNWMCYRKTEDFFTIWLDLNMFLPLGVDCWIDNTRVVYNRSSGLVSNAPGVQIRVPGFGKTYSVEYLDSSKLAGYLHTLVQNLVNNGYVRDETVRAAPYDWRLEPGQQEEYYRKLAGLVEEMHAAYGKPVFLIGHSLGCLHLLYFLLRQPQAWKDRFIDGFISLGAPWGGSIKPMLVLASGDNQGIPIMSSIKLKEEQRITTTSPWMFPSRMAWPEDHVFISTPSFNYTGRDFQRFFADLHFEEGWYMWLQSRDLLAGLPAPGVEVYCLYGVGLPTPRTYIYDHGFPYTDPVGVLYEDGDDTVATRSTELCGLWQGRQPQPVHLLPLHGIQHLNMVFSNLTLEHINAILLGAYRQGPPASPTASPEPPPPE

Approximately 71 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 4 mM HCl.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.