Lck 蛋白, Human (sf9, GST)

The Lck protein is a non-receptor tyrosine protein kinase that is essential for T cell development and function. Lck is constitutively associated with CD4 and CD8 receptors and is recruited to the TCR/CD3 complex following TCR interaction with MHC. Lck Protein, Human (sf9, GST) is the recombinant human-derived Lck protein, expressed by Sf9 insect cells , with N-GST labeled tag. The total length of Lck Protein, Human (sf9, GST) is 509 a.a., with molecular weight of ~80 kDa.

Lck 蛋白是一种非受体酪氨酸蛋白激酶，在胸腺中发育中 T 细胞的选择和成熟以及成熟 T 细胞的功能中发挥着至关重要的作用。它是 T 细胞抗原受体 (TCR) 相关信号转导途径的关键参与者，与 CD4 和 CD8 表面受体的细胞质部分组成性相关。TCR 与肽抗原结合的 MHC 复合物相互作用后，CD4 和 CD8 分别与 MHC II 类和 I 类分子结合，将 Lck 募集到 TCR/CD3 复合物附近。然后，Lck 磷酸化 TCR-γ 链和 CD3 亚基的免疫受体酪氨酸激活基序 (ITAM) 内的酪氨酸残基，启动 TCR/CD3 信号通路。受到刺激后，TCR 会招募酪氨酸激酶 ZAP70，从而产生淋巴因子。Lck 还有助于其他受体的信号传导，直接与 CD2 的细胞质尾部结合，导致 Lck 过度磷酸化和激活。此外，Lck 在 IL2 受体相关信号通路中发挥作用，其活性随着 IL2 与其受体结合而增加，从而影响 T 细胞增殖反应。Lck 在胸腺细胞发育的所有阶段表达，对于调节由前 TCR 和成熟 αβ TCR 控制的成熟事件至关重要，它磷酸化各种底物，包括 RUNX3、PTK2B/PYK2、MAPT、RHOH、TYROBP，并与 FYB2 相互作用。

Measured by its ability to catalyze the substrate Poly(Glu,Tyr) 4:1. The specific activity is ≥35.82 nmol/min/mg, as measured under the described conditions.

Human

Sf9 insect cells

N-GST

P06239 (M1-P509)

3932  [NCBI]

Approximately 80-85 kDa

Greater than 90% as determined by reducing SDS-PAGE

溶液

Supplied as a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, pH 8.0, 0.5 mM GSH, 0.1 mM EGTA, 0.1 mM EDTA, 0.5 mM PMSF, 10% glycerol or 50 mM Tris-HCL, 150 mM NaCl, pH 7.5, 1mM DTT, 20% Glycerol.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.