1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. Legumain 蛋白, Pig (416a.a, HEK293, His)

Legumain 蛋白, Pig (416a.a, HEK293, His)

目录号: HY-P77438
COA 产品使用指南

Legumain 蛋白可以缓慢裂解天冬氨酰键,尤其是在酸性条件下。Legumain 蛋白, Pig (416a.a, HEK293, His) 是重组的 Legumain 蛋白,由 HEK293 表达,带有 N-His 标签。Legumain 蛋白, Pig (416a.a, HEK293, His) 全长 416 个氨基酸,分子量约为 ~52.5 kDa。

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50 μg ¥3800 In-stock
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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

Legumain proteins can slowly cleave aspartyl bonds, especially under acidic conditions. Legumain Protein, Pig (416a.a, HEK293, His) is the recombinant pig-derived Legumain protein, expressed by HEK293 , with N-His labeled tag. The total length of Legumain Protein, Pig (416a.a, HEK293, His) is 416 a.a., with molecular weight of ~52.5 kDa.

研究背景

Legumain 蛋白对天冬酰胺键的水解表现出严格的特异性。此外,它还表现出缓慢裂解天冬氨酰键的能力,特别是在酸性条件下,进一步扩展了其酶的多功能性。从功能上讲,Legumain 是溶酶体/内体系统内 MHC II 类抗原呈递蛋白质加工不可或缺的一部分。最近的研究结果表明,它还通过促进 Perforin-2 (MPEG1) 的裂解和成熟,从而促进细胞质中的抗原易位,从而在交叉呈递树突状细胞的 MHC I 类抗原呈递中发挥关键作用。此外,Legumain 对于肾近曲小管中正常溶酶体蛋白降解至关重要,并且是内化 EGFR 降解所必需的,这凸显了其在细胞过程和细胞增殖调节中的重要性。

生物活性

Measured by its ability to cleave the fluorogenic peptide substrate, N-carbobenzyloxy-Ala-Ala-Asn-7-amido-4-methylcoumarin (Z-AAN-AMC). The specific activity is 2781.650 pmoL/min/µg, as measured under the described conditions.

种属

Pig

表达系统

HEK293

标签

N-6*His

蛋白编号

XP_001927117 (V18-Y433)

基因 ID
蛋白结构
N-term
His
Legumain (V18-Y433)
Accession # XP_001927117
C-term
同用名
Legumain; Asparaginyl Endopeptidase; Protease Cysteine 1; LGMN; PRSC1
氨基酸序列

VPVDDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQIVHRNGIPDEQIIVMMYDDIANSEDNPTPGIVINRPNGSDVYKGVLKDYTGEDVTPQNFLAVLRGDAEAVKGKGSGKVLKSGPRDHVFVYFTDHGATGILVFPNEDLHVKDLNETIHYMYKHRMYQKMVFYIEACESGSMMNHLPPNIDVYATTAANPRESSYACYYDEARATYLGDWYSVNWMEDSDSEDLTRETLHKQYQLVKSHTNTSHVMQYGNKSISAMKLVQFQGLKHKASSPISLPPVQHLDLTPSPEVPLTIMKRKLTRTNDLQESRRLVAEIDRHLQARNVIEKSVRKIVSLTVGSDAEVDRLLSQRDPLTAHECYQEAVLHFRTHCFNWHSPTYEYALRHLYVLANLCENSYPIASIKLSMDKVCLGYY

分子量

Approximately 57 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCL, 50 mM NaCl, pH 8.0.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Legumain Protein, Pig (416a.a, HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Legumain Protein, Pig (416a.a, HEK293, His)
目录号:
HY-P77438
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