LILRB4/CD85k/ILT3 蛋白, Cynomolgus (HEK293, His)

The LILRB4/CD85k/ILT3 protein is a multifaceted inhibitory receptor in immune regulation that significantly downregulates multiple immune responses. As a receptor for FN1 and integrin ITGAV/ITGB3, LILRB4/ILT3 inhibits IgE-mediated mast cell activation, KITLG/SCF-mediated mast cell activation, and antibody production by memory/marginal zone B cells through the ITGAV/ITGB3 interaction. LILRB4/CD85k/ILT3 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived LILRB4/CD85k/ILT3 protein, expressed by HEK293 , with C-His labeled tag. The total length of LILRB4/CD85k/ILT3 Protein, Cynomolgus (HEK293, His) is 238 a.a., with molecular weight of 35-42 kDa.

LILRB4/CD85k/ILT3 是一种与免疫调节密切相关的抑制性受体，在下调免疫反应中发挥着至关重要的作用。它作为 FN1 和整合素 ITGAV/ITGB3 的受体，对 IgE 介导的肥大细胞激活和 KITLG/SCF 介导的肥大细胞激活发挥抑制作用。通过与 ITGAV/ITGB3 的相互作用，LILRB4/ILT3 进一步抑制记忆和边缘区 B 细胞的抗体产生，可能是通过抑制它们分化为浆细胞。这种多面受体将其抑制影响扩展到多种免疫功能，例如抑制 CD8 T 细胞、CD4 T 细胞和自然杀伤细胞产生 IFNG，以及抑制树突状细胞向 T 细胞呈递抗原，防止 T 细胞活化。此外，LILRB4/ILT3 可有效抑制脂多糖介导的中性粒细胞依赖性血管损伤，并通过阻止中性粒细胞和嗜酸性粒细胞浸润，同时防止肥大细胞脱颗粒，有助于抑制过敏性炎症反应。它的相互作用，特别是当酪氨酸磷酸化时，与含有 SH2 结构域的磷酸酶 PTPN6/SHP-1 和 PTPN11/SHP-2 的相互作用增强了对肥大细胞活化的抑制。

Immobilized Cynomolgus LILRB4,His Tag at 5 ug/mL(100 μL/well) on the plate. Dose response curve for Anti-LILRB4 Antibody,hFc Tag with the EC₅₀ of 0.13-0.38 μg/mL determined by ELISA.

Cynomolgus

HEK293

C-His

XP_015297198.1 (Q22-E259)

107128256  [NCBI]

35-42 kDa

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.