Lipoate-protein ligase A/LplA 蛋白, E.coli (His-SUMO)

Lipoic acid protein ligase A (LplA) is a key enzyme involved in the activation and transfer of lipoic acid, an important cofactor, to the lipoyl domain of lipoic acid-dependent enzymes. LplA catalyzes the ATP-dependent activation of exogenous lipoic acid, converting it to lipoyl-AMP as an intermediate. Lipoate-protein ligase A/LplA Protein, E.coli (His-SUMO) is the recombinant E. coli-derived Lipoate-protein ligase A/LplA protein, expressed by E. coli , with N-SUMO, N-6*His labeled tag. The total length of Lipoate-protein ligase A/LplA Protein, E.coli (His-SUMO) is 338 a.a., with molecular weight of ~53.9 kDa.

硫辛酸蛋白连接酶 A (LplA) 是一种关键酶，参与硫辛酸（一种重要的辅助因子）的激活和转移到硫辛酸依赖性酶的硫辛酰结构域上。LplA 催化外源硫辛酸的 ATP 依赖性激活，将其转化为硫辛酰-AMP，作为中间体。然后，LplA 利用这种活化的硫辛酰-AMP 将硫辛酰部分转移到靶蛋白上。通过促进这一转移过程，LplA 确保硫辛酸依赖性酶的正常功能，这些酶参与各种重要的代谢途径，例如柠檬酸循环和脂肪酸代谢。了解 LplA 介导的硫辛酸活化和转移的机制和调节可以为细胞能量代谢和代谢稳态的维持提供见解。

E.coli

E. coli

N-SUMO;N-6*His

C4ZT68 (M1-R338)

/

MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTVVIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTISTSIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLSRLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITEAFFAHYGERVEAEIISPNKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFTWGGVELHFDVEKGHITRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPEQEKELRELSAWMAGAVR

Approximately 53.9 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.