Lipocalin-2/NGAL 蛋白, Cynomolgus (CHO, His)

Lipocalin-2/NGAL is a multifaceted iron transporter involved in multiple biological processes, including apoptosis, innate immunity, and kidney development. Lipocalin-2 dynamically affects cellular iron concentration through binding to the siderophore 2,3-dihydroxybenzoate. Lipocalin-2/NGAL Protein, Cynomolgus (CHO, His) is the recombinant cynomolgus-derived Lipocalin-2/NGAL protein, expressed by CHO , with C-His labeled tag.

Lipocalin-2/NGAL 是一种多方面的铁转运蛋白，参与多种生物过程，包括细胞凋亡、先天免疫和肾脏发育。通过与铁载体 2,3-二羟基苯甲酸 (2,3-DHBA) 的结合，Lipocalin-2 结合并穿梭铁，动态影响基于 Holo-24p3（铁结合）或 apo-24p3（铁）的细胞铁浓度。-免费）表格。与 SLC22A17 受体的相互作用介导铁释放或螯合，精细调节细胞内铁水平。在白细胞介素 3 (IL3) 剥夺引发的细胞凋亡中，含铁形式可防止细胞凋亡，而无铁形式则诱导 BCL2L11/BIM 表达，导致程序性细胞死亡。Lipocalin-2 还通过隔离细菌铁载体（例如肠杆菌素）来促进先天免疫，并表现出结合结核分枝杆菌铁载体的能力。在结构上，Lipocalin-2 以单体、同二聚体（二硫键连接）形式存在，并与 MMP9 形成异二聚体（二硫键连接）。这些不同的相互作用强调了 Lipocalin-2 在协调关键细胞和免疫反应方面的多功能性。

Measured by its ability to bind Iron(III) dihydroxybenzoic acid [Fe(DHBA)3] in 30 min at 25 ℃. The binding of Fe(DHBA)3 results in the quenching of Trp fluorescence in 2 μM NGAL. 1.86 μM of Fe(DHBA)3 can be bound.

Cynomolgus

CHO

C-6*His

XP_005580845.2 (Q21-G198)

102137149  [NCBI]

QDSSSDLIPAPPLSKVPLQQNFQDNQFQGKWYVVGLSGNAVGRKDEAPLKMYATIYELKEDKSYNVTSILFRKEKCDYWIRTFVPGSQPGEFTLGNIQNHPGLTSYVVRVVSTNYKQYAMVFFKKVSQNKEYFKITLYGRTKELTSELKENFIRFSKSLGLPENHIVFSVPIDQCIDG

Approximately 22-23 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<0.1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.