1. 重组蛋白
  2. Enzymes & Regulators
  3. Oxidoreductases (EC 1)
  4. lipoxygenase 蛋白, Pseudomonas aeruginosa

lipoxygenase 蛋白, Pseudomonas aeruginosa

目录号: HY-P702170
产品使用指南

脂氧合酶蛋白催化氧介导的亚油酸转化为9HPOD,并自发分解为9HOD。它将亚麻酸转化为 13HPOD,13HPOD 又分解为 13HOD。lipoxygenase 蛋白, Pseudomonas aeruginosa 是重组的 脂氧合酶蛋白,由 E. coli 表达,不带标签。lipoxygenase 蛋白, Pseudomonas aeruginosa 全长 678 个氨基酸。

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描述

The lipoxygenase protein catalyzes the oxygen-mediated conversion of linoleic acid to 9HPOD and spontaneously decomposes it into 9HOD. It converts linolenic acid into 13HPOD, which breaks down into 13HOD. lipoxygenase Protein, Pseudomonas aeruginosa is the recombinant lipoxygenase protein, expressed by E. coli , with tag free. The total length of lipoxygenase Protein, Pseudomonas aeruginosa is 678 a.a., .

研究背景

在氧气存在下,脂氧合酶蛋白催化亚油酸酯转化为 (9S)-氢过氧-10,12-十八烯酸酯 (9HPOD),随后自发分解为相应的 9-羟基-10,12-十八烯酸酯 (9HOD)。它还将亚麻酸酯转化为其氢过氧衍生物,13-氢过氧-9,11-十八碳烯酸酯(13HPOD),后者自发分解为相应的13-羟基-9,11-十八碳烯酸酯(13HOD)。此外,该酶对油酸表现出活性(尽管程度较低),将其转化为 (10S)-氢过氧-8E-十八烯酸,其自发分解形成相应的 10-羟基-8E-十八烯酸。值得注意的是,脂氧合酶对花生四烯酸的活性极低。这种酶的多功能性凸显了其在介导多种脂肪酸氧合反应中的作用,有助于与脂质信号传导和炎症相关的复杂代谢途径。

种属

Others

表达系统

E. coli

标签

Tag Free

蛋白编号

Q8RNT4 (M1-I678)

基因 ID

/

蛋白结构
N-term
lipoxygenase (M1-I678)
Accession # Q8RNT4
C-term
同用名
lox; Linoleate 9/13-lipoxygenase; Oleate 10S-lipoxygenase
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

lipoxygenase Protein, Pseudomonas aeruginosa 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
lipoxygenase Protein, Pseudomonas aeruginosa
目录号:
HY-P702170
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