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  3. LolA 蛋白, E.coli (P.pastoris, His)

LolA 蛋白, E.coli (P.pastoris, His)

目录号: HY-P71812
产品使用指南

LolA 蛋白将脂蛋白从细菌内膜转移到外膜,形成依赖于 N 末端半胱氨酸后天冬氨酸缺失的复合物。天冬氨酸向脂蛋白发出信号,使其留在内膜中。LolA 作为单体,可以有效地在细菌膜区室之间移动脂蛋白。LolA 蛋白, E.coli (P.pastoris, His) 是重组的 LolA 蛋白,由 P. pastoris 表达,带有 N-His 标签。LolA 蛋白, E.coli (P.pastoris, His) 全长 182 个氨基酸,分子量约为 ~22.3 kDa。

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描述

LolA Protein translocates lipoproteins from bacterial inner to outer membranes, forming a complex dependent on the absence of aspartate after the N-terminal cysteine. Aspartate signals lipoproteins to stay in the inner membrane. LolA, as a monomer, efficiently moves lipoproteins between bacterial membrane compartments. LolA Protein, E.coli (P.pastoris, His) is the recombinant E. coli-derived LolA protein, expressed by P. pastoris , with N-His labeled tag.

研究背景

LolA 蛋白参与细菌细胞中脂蛋白从内膜到外膜的易位。它与脂蛋白形成复合物,但这种相互作用取决于脂蛋白序列中 N 末端半胱氨酸紧随其后不存在天冬氨酸。天冬氨酸的存在充当靶向信号,表明脂蛋白应保留在内膜中。LolA 作为单体促进脂蛋白在细菌膜区室之间有效移动。

种属

E.coli

表达系统

P. pastoris

标签

N-His

蛋白编号

A7ZYJ5 (D22-K203)

基因 ID

/

蛋白结构
N-term
His
LolA (D22-K203)
Accession # A7ZYJ5
C-term
同用名
lolA; Outer-membrane lipoprotein carrier protein
氨基酸序列

DAASDLKSRLDKVSSFHASFTQKVTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWLKDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQFSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK

分子量

Approximately 22.3 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

LolA Protein, E.coli (P.pastoris, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
LolA Protein, E.coli (P.pastoris, His)
目录号:
HY-P71812
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