Lon Protease 蛋白, E. coli (His)

Lon protease is an ATP-dependent serine protease that selectively degrades mutant proteins, short-lived regulators, and specific antitoxins and is critical for cellular homeostasis. It is essential for survival during DNA damage and stress-induced changes by gradually degrading polypeptides into 5-10 amino acid fragments. Lon Protease Protein, E. coli (His) is the recombinant E. coli-derived Lon Protease protein, expressed by E. coli , with C-His labeled tag. The total length of Lon Protease Protein, E. coli (His) is 784 a.a., with molecular weight of ~88 kDa.

Lon 蛋白酶是一种 ATP 依赖性丝氨酸蛋白酶，在突变和异常蛋白以及某些短寿命调节蛋白（包括特定抗毒素）的选择性降解中充当关键介质。Lon 蛋白酶对于细胞稳态至关重要，在确保 DNA 损伤和应激诱导的发育变化条件下的生存方面发挥着关键作用。通过持续操作，该蛋白酶降解多肽，生成长度通常为 5 至 10 个氨基酸的小肽片段。此外，Lon 蛋白酶还具有双链、位点特异性的 DNA 结合能力。其内源性底物包括调节蛋白（如 RcsA、SulA、SoxS、UmuD）和各种 II 型抗毒素（如 CcdA、HipB 和 MazE）。值得注意的是，Lon 蛋白酶在过量产生时，会选择性地阻碍通过不同途径的翻译，包括 YoeB-YefM 毒素-抗毒素系统。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

E.coli

E. coli

C-His

P0A9M0 (M1-K784)

945085  [NCBI]

Approximately 88 kDa

Greater than 90% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, 10% Glycerol, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.