Lon Protease 蛋白, E. coli (His)

Lon Protease, an ATP-dependent serine protease, selectively degrades mutant proteins, short-lived regulators, and specific antitoxins, vital for cellular homeostasis. Crucial for survival during DNA damage and stress-induced changes, it processively degrades polypeptides into 5-10 amino acid fragments. Lon Protease also exhibits site-specific, double-stranded DNA binding. Its substrates include RcsA, SulA, SoxS, UmuD, and type II antitoxins like CcdA, HipB, and MazE. Notably, overproduced Lon Protease selectively hinders translation through pathways, including the YoeB-YefM toxin-antitoxin system. Lon Protease Protein, E. coli (His) is the recombinant E. coli-derived Lon Protease protein, expressed by E. coli, with C-His labeled tag. The total length of Lon Protease Protein, E. coli (His) is 784 a.a., with molecular weight of ~88 kDa.

Lon 蛋白酶是一种 ATP 依赖性丝氨酸蛋白酶，在突变和异常蛋白以及某些短寿命调节蛋白（包括特定抗毒素）的选择性降解中充当关键介质。Lon 蛋白酶对于细胞稳态至关重要，在确保 DNA 损伤和应激诱导的发育变化条件下的生存方面发挥着关键作用。通过持续操作，该蛋白酶降解多肽，生成长度通常为 5 至 10 个氨基酸的小肽片段。此外，Lon 蛋白酶还具有双链、位点特异性的 DNA 结合能力。其内源性底物包括调节蛋白（如 RcsA、SulA、SoxS、UmuD）和各种 II 型抗毒素（如 CcdA、HipB 和 MazE）。值得注意的是，Lon 蛋白酶在过量产生时，会选择性地阻碍通过不同途径的翻译，包括 YoeB-YefM 毒素-抗毒素系统。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

E.coli

E. coli

C-His

P0A9M0 (M1-K784)

61752336

Approximately 88 kDa

Greater than 90% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, 10% Glycerol, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.