LRP-5 蛋白, Human (HEK293, mFc)

LRP-5 Protein functions as a coreceptor, collaborating with frizzled receptors to initiate the canonical Wnt signaling pathway. It crucially regulates cell fate determination, self-renewal, and plays a significant role in posterior epiblast patterning during gastrulation and bone development by influencing osteoblast proliferation and differentiation. LRP-5 facilitates the formation of a signaling complex, stabilizing beta-catenin and activating TCF/LEF-mediated transcription. As a coreceptor for non-Wnt proteins like norrin/NDP, LRP-5 contributes to retinal vascular development. Interactions with partners, including FZD8, AXIN1, DKK1, MESD, KREMEN2, CSNK1E, SOST, APCDD1, and CAPRIN2, underscore its intricate involvement in diverse cellular processes. LRP-5 Protein, Human (HEK293, mFc) is the recombinant human-derived LRP-5 protein, expressed by HEK293, with C-mFc labeled tag. The total length of LRP-5 Protein, Human (HEK293, mFc) is 620 a.a., with molecular weight of 110-115 kDa.

LRP-5 蛋白充当关键的辅助受体，与卷曲的七次跨膜受体家族合作，转导由 Wnt 蛋白启动的信号。这种激活导致经典的 Wnt 信号通路，这是胚胎发育和成体组织再生过程中细胞命运决定和自我更新的关键调节因子。LRP-5 尤其与原肠胚形成过程中外胚层的后部模式有关，并通过调节成骨细胞增殖和分化在骨发育中发挥至关重要的作用。从机制上讲，LRP-5 促进 Wnt 配体、卷曲受体及其自身之间信号传导复合物的形成，从而导致 AXIN1 的募集。这可以稳定 β-catenin/CTNNB1 并激活 TCF/LEF 介导的转录程序。此外，LRP-5 作为非 Wnt 蛋白（例如 Norrin/NDP）的辅助受体，在视网膜血管发育中具有已被证明的作用。与各种伙伴（包括 FZD8、AXIN1、DKK1、MESD、KREMEN2、CSNK1E、SOST、APCDD1 和 CAPRIN2）的相互作用凸显了其在多种细胞过程调节中的复杂参与。

Immobilized Human DKK1, His Tag at 5 μg/mL (100μl/well) on the plate. Dose response curve for Human LRP-5, mFc Tag with the EC₅₀ of 0.11 μg/mL determined by ELISA.

Human

HEK293

C-mFc

O75197 (E644-Q1263)

4041  [NCBI]

110-115 kDa

Greater than 90% as determined by Tris-Bis PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.