LRPAP1 蛋白, Human (HEK293, C-His)

LRPAP1 protein functions as a molecular chaperone for LDL receptor-related proteins, regulating ligand binding throughout the secretory pathway. It transiently interacts with LRP1/alpha-2-macroglobulin receptor chains, reducing ligand binding. LRPAP1 also interacts with LRP2/glycoprotein 330, LDLR, and SORL1, influencing internalization, degradation, or ligand binding modulation. This highlights LRPAP1's multifaceted role in governing the function and trafficking of LDL receptor-related proteins. LRPAP1 Protein, Human (HEK293, C-His) is the recombinant human-derived LRPAP1 protein, expressed by HEK293, with C-6*His labeled tag. The total length of LRPAP1 Protein, Human (HEK293, C-His) is 323 a.a., with molecular weight of ~42 KDa.

LRPAP1 蛋白作为 LDL 受体相关蛋白的分子伴侣，有助于在整个分泌途径中调节其配体结合活性。值得注意的是，LRPAP1 与 LRP1/α-2-巨球蛋白受体重链和轻链发生短暂的相互作用，与受体配体结合的减少同时发生。此外，LRPAP1 与 LRP2/糖蛋白 330、LDLR 和 SORL1 形成相互作用，产生从内化和降解到调节配体结合等多种结果。复杂的相互作用网络强调了 LRPAP1 在协调 LDL 受体相关蛋白的功能和运输中的多方面作用。

Immobilized LRPAP1 at 0.5 μg/mL (100 μL/well) can bind Biotinylated VLDLR protein. The ED₅₀ for this effect is 0.5626 nM.

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  Immobilized LRPAP1 at 0.5 μg/mL (100 μL/well) can bind Biotinylated VLDLR protein. The ED₅₀ for this effect is 0.5626 nM.

Human

HEK293

C-6*His

P30533 (Y35-L357)

4043

YSREKNQPKPSPKRESGEEFRMEKLNQLWEKAQRLHLPPVRLAELHADLKIQERDELAWKKLKLDGLDEDGEKEARLIRNLNVILAKYGLDGKKDARQVTSNSLSGTQEDGLDDPRLEKLWHKAKTSGKFSGEELDKLWREFLHHKEKVHEYNVLLETLSRTEEIHENVISPSDLSDIKGSVLHSRHTELKEKLRSINQGLDRLRRVSHQGYSTEAEFEEPRVIDLWDLAQSANLTDKELEAFREELKHFEAKIEKHNHYQKQLEIAHEKLRHAESVGDGERVSRSREKHALLEGRTKELGYTVKKHLQDLSGRISRARHNEL

Approximately 42 kDa.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.