LSD1 蛋白, Human (sf9, His-GST)

The LSD1 protein is a histone demethylase that modifies histone H3 by demethylating “Lys-4” (H3K4me) and “Lys-9” (H3K9me).It acts as a coactivator or corepressor, oxidizing substrates via FAD to generate imines that are subsequently hydrolyzed.LSD1 Protein, Human (sf9, His-GST) is the recombinant human-derived LSD1 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.

LSD1 是一种组蛋白去甲基化酶，具有使组蛋白 H3 的“Lys-4”(H3K4me) 和“Lys-9”(H3K9me) 去甲基化的独特能力，根据细胞环境充当多功能共激活子或辅阻遏物。通过 FAD 介导的氧化和随后的亚胺水解，LSD1 可以使单甲基化和二甲基化的 H3K4me 去甲基化，从而有助于表观遗传转录激活的调节。在缺乏 RCOR1/CoREST 的情况下，单独使用 LSD1 无法使核小体上的 H3K4me 去甲基化。此外，LSD1 作为雄激素受体 (AR) 依赖性转录的共激活剂，参与 H3K9me（与表观遗传转录抑制相关的标记物）的去甲基化。此外，LSD1 在抑制神经元基因中发挥着关键作用，并通过参与 RCOR/GFI/KDM1A/HDAC 复合物协调 E-钙粘蛋白/CDH1、CDN7 和 KRT8 的抑制。除了组蛋白去甲基化之外，LSD1 的多种相互作用还延伸到 p53/TP53 去甲基化、稳定 DNMT1 和修饰 SOX2。LSD1 的复杂调节作用涵盖胚胎发生、原肠胚形成和多谱系血细胞发育，强调其在塑造表观遗传景观和影响各种细胞过程中的重要性。

LSD1 demethylates H3K4 to produce formaldehyde, and then in the presence of ammonia, formaldehyde reacts with acetyl linear aniline to produce fluorescent products. The specific activity is 36.565 ng/min/mg.

Human

Sf9 insect cells

N-His;N-GST

O60341-1 (S172-M852)

23028  [NCBI]

SGVEGAAFQSRLPHDRMTSQEAACFPDIISGPQQTQKVFLFIRNRTLQLWLDNPKIQLTFEATLQQLEAPYNSDTVLVHRVHSYLERHGLINFGIYKRIKPLPTKKTGKVIIIGSGVSGLAAARQLQSFGMDVTLLEARDRVGGRVATFRKGNYVADLGAMVVTGLGGNPMAVVSKQVNMELAKIKQKCPLYEANGQAVPKEKDEMVEQEFNRLLEATSYLSHQLDFNVLNNKPVSLGQALEVVIQLQEKHVKDEQIEHWKKIVKTQEELKELLNKMVNLKEKIKELHQQYKEASEVKPPRDITAEFLVKSKHRDLTALCKEYDELAETQGKLEEKLQELEANPPSDVYLSSRDRQILDWHFANLEFANATPLSTLSLKHWDQDDDFEFTGSHLTVRNGYSCVPVALAEGLDIKLNTAVRQVRYTASGCEVIAVNTRSTSQTFIYKCDAVLCTLPLGVLKQQPPAVQFVPPLPEWKTSAVQRMGFGNLNKVVLCFDRVFWDPSVNLFGHVGSTTASRGELFLFWNLYKAPILLALVAGEAAGIMENISDDVIVGRCLAILKGIFGSSAVPQPKETVVSRWRADPWARGSYSYVAAGSSGNDYDLMAQPITPGPSIPGAPQPIPRLFFAGEHTIRNYPATVHGALLSGLREAGRIADQFLGAMYTLPRQATPGVPAQQSPSM

Approximately 93 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, 2 mM DTT, pH 8.0, 10% Glycerol . Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.