LSM3 蛋白, Human (His)

The LSM3 protein plays a crucial role in pre-mRNA splicing as a component of the U4/U6-U5 tri-snRNP complex involved in spliceosome assembly and as part of the precatalytic spliceosome (spliceosome B complex). The heptameric LSM2-8 complex (including LSM3) specifically binds to the 3'-end U strand of U6 snRNA. LSM3 Protein, Human (His) is the recombinant human-derived LSM3 protein, expressed by E. coli , with N-His labeled tag.

LSM3 蛋白作为 U4/U6-U5 tri-snRNP 复合物的重要组成部分，在前 mRNA 剪接中发挥着关键作用，参与剪接体组装和预催化剪接体（剪接体 B 复合物）。LSM3 是七聚体 LSM2-8 复合物的组成部分，该复合物特异性结合 U6 snRNA 的 3' 端 U 链。这种复杂的机制由各种 snRNP 和相关蛋白（包括 LSM3、LSM2、LSM4、LSM5、LSM6、LSM7 和 LSM8）组成，在 U4/U6-U5 tri-snRNP 复合体中形成环形子复合体。这些相互作用强调了 LSM3 对剪接体组装和前 mRNA 加工的复杂协调的重要贡献。

Human

E. coli

N-His

P62310 /NP_055278.1(M1-G102)

27258  [NCBI]

MADDVDQQQTTNTVEEPLDLIRLSLDERIYVKMRNDRELRGRLHAYDQHLNMILGDVEETVTTIEIDEETYEEIYKSTKRNIPMLFVRGDGVVLVAPPLRVG

Approximately 16 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.