LUXS 蛋白, E.coli (His)

LUXS proteins are critical for the synthesis of bacterially secreted autoinducer 2 (AI-2), which promotes communication between cell density and environmental metabolism. LUXS is critical for quorum sensing and catalyzes S-ribosylhomocysteine (RHC) to homocysteine (HC) and 4,5-dihydroxy-2,3-pentanedione (DPD) , revealing its enzymatic role in AI-2 production. LUXS Protein, E.coli (His) is the recombinant E. coli-derived LUXS protein, expressed by E. coli , with N-6*His labeled tag. The total length of LUXS Protein, E.coli (His) is 170 a.a., with molecular weight of 23-25 kDa.

LUXS 蛋白在自诱导剂 2 (AI-2) 的合成中发挥着关键作用，自诱导剂 2 (AI-2) 是细菌分泌的一种信号分子，可促进细胞密度和环境代谢潜力方面的通讯。这个过程是群体感应不可或缺的一部分，群体感应是根据细胞密度的变化来调节基因表达。LUXS 催化 S-核糖基高半胱氨酸 (RHC) 转化为高半胱氨酸 (HC) 和 4,5-二羟基-2,3-戊二酮 (DPD)，阐明其酶促参与 AI-2 的产生。LUXS 的复杂功能凸显了其在细菌通讯和传感机制中的关键作用，有助于协调细菌行为以响应环境线索。

E.coli

E. coli

N-6*His

P45578 (P2-I171)

947168  [NCBI]

PLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPNKEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVADAWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELALPKEKLQELHI

23-25 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against solution in PBS, 6% Trehalose, pH 7.4 or PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.