1. 重组蛋白
  2. Cytokines and Growth Factors
  3. CSF & Receptors
  4. M-CSF
  5. M-CSF 蛋白, Human (Tag free, HEK293)

M-CSF 蛋白, Human (Tag free, HEK293)

目录号: HY-P701251
产品使用指南

M-CSF蛋白是一种重要的细胞因子,可调节造血前体细胞,特别是单核吞噬细胞,通过释放促炎趋化因子影响先天免疫和炎症。它对于破骨细胞增殖至关重要,并调节骨吸收、骨发育和生育能力。M-CSF 蛋白, Human (Tag free, HEK293) 是重组的 M-CSF 蛋白,由 HEK293 表达,不带标签。M-CSF 蛋白, Human (Tag free, HEK293) 全长 158 个氨基酸,糖基化后的分子量为 23-28 kDa。

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描述

The M-CSF protein is an important cytokine that modulates hematopoietic precursor cells, especially mononuclear phagocytes, affecting innate immunity and inflammation through the release of proinflammatory chemokines. It is essential for osteoclast proliferation and regulates bone resorption, bone development and fertility. M-CSF Protein, Human (Tag free, HEK293) is the recombinant human-derived M-CSF protein, expressed by HEK293 , with tag free. The total length of M-CSF Protein, Human (Tag free, HEK293) is 158 a.a., with molecular weight (glycosylation form) of 23-28 kDa.

研究背景

M-CSF 蛋白是一种重要的细胞因子,参与调节造血前体细胞(特别是巨噬细胞和单核细胞等单核吞噬细胞)的存活、增殖和分化。它通过促进促炎趋化因子的释放在先天免疫和炎症过程中发挥至关重要的作用。此外,M-CSF 蛋白对于破骨细胞增殖和分化、调节骨吸收和正常骨骼发育至关重要。它也是正常男性和女性生育能力所必需的。此外,M-CSF 蛋白有助于肌动蛋白细胞骨架的重组,促进膜皱褶形成、细胞粘附和细胞迁移。此外,它在脂蛋白清除中发挥作用。M-CSF 蛋白可以不同的形式存在,例如同二聚体或异二聚体构型,并且它与 CSF1R 相互作用。

种属

Human

表达系统

HEK293

标签

Tag Free

蛋白编号

P09603-1 (E33-N190)

基因 ID

1435

蛋白结构
N-term
M-CSF (E33-N190)
Accession # P09603-1
C-term
同用名
Macrophage Colony-Stimulating Factor 1; CSF-1; M-CSF; Lanimostim
氨基酸序列

EEVSEYCSHMIGSGHLQSLQRLIDSQMETSCQITFEFVDQEQLKDPVCYLKKAFLLVQDIMEDTMRFRDNTPNAIAIVQLQELSLRLKSCFTKDYEEHDKACVRTFYETPLQLLEKVKNVFNETKNLLDKDWNIFSKNCNNSFAECSSQDVVTKPDCN

分子量

23-28 kDa, due to glycosylation

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

< 1 EU/μg of protein by gel clotting method

文件资料

M-CSF Protein, Human (Tag free, HEK293) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
M-CSF Protein, Human (Tag free, HEK293)
目录号:
HY-P701251
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