2. 重组蛋白
  3. Enzymes & Regulators
  4. Transferases (EC 2)
  5. MAK 蛋白, Human (Sf9, His, GST)

MAK 蛋白, Human (Sf9, His, GST)

目录号: HY-P701797
产品使用指南

MAK 蛋白对于纤毛长度调节至关重要，它通过以细胞周期依赖性方式磷酸化 FZR1 来确保光感受器的存活。它共同激活 AR 转录并可能有助于精子发生，并可能在维持前列腺癌细胞染色体稳定性方面发挥作用。MAK 蛋白, Human (Sf9, His, GST) 是重组的 MAK 蛋白，由 Sf9 insect cells 表达，带有 N-8*His, N-GST 标签。MAK 蛋白, Human (Sf9, His, GST) 全长 622 个氨基酸。

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描述

MAK Protein, vital for ciliary length regulation, ensures photoreceptor survival by phosphorylating FZR1 in a cell cycle-dependent manner. It coactivates AR transcription and potentially aids spermatogenesis, with a possible role in maintaining chromosomal stability in prostate cancer cells. MAK Protein, Human (Sf9, His, GST) is the recombinant human-derived MAK protein, expressed by Sf9 insect cells , with N-8*His, N-GST labeled tag. The total length of MAK Protein, Human (Sf9, His, GST) is 622 a.a., .
研究背景

MAK 蛋白对于纤毛长度的调节至关重要，并且对于光感受器的延长存活至关重要。它以细胞周期依赖性方式磷酸化 FZR1，并参与 AR 的转录共激活。此外，MAK 可能有助于精子发生，并在维持前列腺癌细胞染色体稳定性方面发挥作用。
种属

Human
表达系统

Sf9 insect cells
标签

N-8*His;N-GST
蛋白编号

P20794 (N2-R623)
基因 ID

4117
蛋白结构
N-term
8*His-GST
MAK (N2-R623)
Accession # P20794
C-term
同用名
MAK; Serine/threonine-protein kinase MAK; Male germ cell-associated kinase
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

MAK Protein, Human (Sf9, His, GST) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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