2. 重组蛋白
  3. Enzymes & Regulators
  4. Transferases (EC 2)
  5. MAP2K4 蛋白, Human (Sf9)

MAP2K4 蛋白是参与 MAP 激酶途径的双特异性激酶。MAP2K4 蛋白, Human (Sf9) 是重组的 MAP2K4 蛋白，由 Sf9 insect cells 表达，不带标签。

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描述

The MAP2K4 protein is a dual-specificity kinase involved in the MAP kinase pathway. MAP2K4 Protein, Human (Sf9) is the recombinant human-derived MAP2K4 protein, expressed by Sf9 insect cells , tag free.
研究背景

MAP2K4 是一种双特异性蛋白激酶，是 MAP 激酶信号转导通路中的关键组件，特别是在应激激活蛋白激酶/c-Jun N 末端激酶 (SAP/JNK) 信号级联中。MAP2K4 与 MAP2K7/MKK7 合作，成为少数已知能够通过 Thr-Pro-Tyr 磷酸化直接激活应激激活蛋白激酶（包括 MAPK8/JNK1、MAPK9/JNK2 和 MAPK10/JNK3）的激酶之一主题。虽然 MAP2K4/MKK4 和 MAP2K7/MKK7 都有助于 JNK 激活，但它们对该基序内磷酸化位点的偏好不同。MAP2K4 有利于 Tyr 残基的磷酸化，而 MAP2K7/MKK7 则有利于 Thr 残基的磷酸化。MAP2K7/MKK7 对 Thr 残基的磷酸化似乎对于 JNK 激活至关重要，尤其是在响应促炎细胞因子时，而其他刺激则使 MAP2K4/MKK4 和 MAP2K7/MKK7 参与 JNK 的协同磷酸化。值得注意的是，MAP2K4 在维持外周淋巴稳态中发挥着重要作用，并参与线粒体死亡信号通路，导致细胞凋亡。MAP2K7/MKK7 专门激活 JNK，而 MAP2K4/MKK4 则更进一步，额外激活 p38 MAPK MAPK11、MAPK12、MAPK13 和 MAPK14。
种属

Human
表达系统

Sf9 insect cells
标签

Tag Free
蛋白编号

P45985-1 (A2-D399)
基因 ID

6416
蛋白结构
N-term
MAP2K4 (A2-D399)
Accession # P45985-1
C-term
同用名
MAP2K4; mitogen-activated protein kinase kinase 4; SERK1; dual specificity mitogen-activated protein kinase kinase 4; JNKK1; MEK4; MKK4; PRKMK4; MEK 4; MAPKK 4; SAPK kinase 1; MAPK/ERK kinase 4; SAPK/ERK kinase 1; MAP kinase kinase 4; JNK-activated kinase 1; JNK activating kinase 1; JNK-activating kinase 1; c-Jun N-terminal kinase kinase 1; stress-activated protein kinase kinase 1; JNKK; SEK1; MAPKK4; SAPKK1; SAPKK-1;
氨基酸序列

AAPSPSGGGGSGGGSGSGTPGPVGSPAPGHPAVSSMQGKRKALKLNFANPPFKSTARFTLNPNPTGVQNPHIERLRTHSIESSGKLKISPEQHWDFTAEDLKDLGEIGRGAYGSVNKMVHKPSGQIMAVKRIRSTVDEKEQKQLLMDLDVVMRSSDCPYIVQFYGALFREGDCWICMELMSTSFDKFYKYVYSVLDDVIPEEILGKITLATVKALNHLKENLKIIHRDIKPSNILLDRSGNIKLCDFGISGQLVDSIAKTRDAGCRPYMAPERIDPSASRQGYDVRSDVWSLGITLYELATGRFPYPKWNSVFDQLTQVVKGDPPQLSNSEEREFSPSFINFVNLCLTKDESKRPKYKELLKHPFILMYEERAVEVACYVCKILDQMPATPSSPMYVD

纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

MAP2K4 Protein, Human (Sf9) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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