1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. MAP2K7 蛋白, Human (Sf9, His, GST)

MAP2K7 蛋白, Human (Sf9, His, GST)

目录号: HY-P701799
产品使用指南

MAP2K7 蛋白是参与 MAP 激酶信号转导途径的双特异性激酶。MAP2K7 蛋白, Human (Sf9, His, GST) 是重组的 MAP2K7 蛋白,由 Sf9 insect cells 表达,带有 N-8*His, N-GST 标签。MAP2K7 蛋白, Human (Sf9, His, GST) 全长 418 个氨基酸。

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描述

MAP2K7 protein is a dual-specificity kinase involved in the MAP kinase signal transduction pathway. MAP2K7 Protein, Human (Sf9, His, GST) is the recombinant human-derived MAP2K7 protein, expressed by Sf9 insect cells , with N-8*His, N-GST labeled tag. The total length of MAP2K7 Protein, Human (Sf9, His, GST) is 418 a.a., .

研究背景

MAP2K7 是一种双特异性蛋白激酶,作为 MAP 激酶信号转导途径的重要组成部分发挥着关键作用,特别是在应激激活蛋白激酶/c-Jun N 末端激酶 (SAP/JNK) 信号级联中。MAP2K7/MKK7 与 MAP2K4/MKK4 合作,通过磷酸化关键的 Thr-Pro-Tyr 基序,直接激活 JNK 家族成员,包括 MAPK8/JNK1、MAPK9/JNK2 和 MAPK10/JNK3。值得注意的是,MAP2K7/MKK7 优先磷酸化 Thr 残基,而 MAP2K4/MKK4 优先磷酸化 Tyr 残基。这种区别确保了最佳的 JNK 激活,因为仅 Thr 残基上的 JNK 单磷酸化就足以增强其活性。MAP2K7/MKK7 特异性参与促炎细胞因子激活的 JNK 信号通路,有助于细胞反应,包括导致细胞凋亡的线粒体死亡信号传导。此外,MAP2K7 与 MAP3K12、MAPK1/ERK2 和 MAPK3/ERK1 一起参与非经典 MAPK 信号通路,增强 AP-1 介导的 APP 转录以响应 APOE。

种属

Human

表达系统

Sf9 insect cells

标签

N-8*His;N-GST

蛋白编号

O14733 (A2-R419)

基因 ID

5609

蛋白结构
N-term
8*His-GST
MAP2K7 (A2-R419)
Accession # O14733
C-term
同用名
MAP2K7; Dual specificity mitogen-activated protein kinase kinase 7; MAP kinase kinase 7; MAPKK 7; JNK-activating kinase 2; MAPK/ERK kinase 7; MEK 7; Stress-activated protein kinase kinase 4; SAPK kinase 4; SAPKK-4; SAPKK4; c-Jun N-terminal kinase kinase 2; JNK kinase 2; JNKK 2
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

MAP2K7 Protein, Human (Sf9, His, GST) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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MAP2K7 Protein, Human (Sf9, His, GST)
目录号:
HY-P701799
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