1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Chemokine & Receptors
  4. CC Chemokines
  5. CCL12
  6. MCP-5/CCL12 蛋白, Mouse (CHO)

MCP-5/CCL12 蛋白, Mouse (CHO)

目录号: HY-P7246
COA 产品使用指南

MCP-5/CCL12 蛋白, Mouse (CHO) 是一种有效的单核细胞活性趋化因子,可以与 CCR2 趋化因子受体结合,参与过敏性炎症和宿主对病原体的反应。MCP-5/CCL12 蛋白, Mouse (CHO)是一种由 CHO 表达的重组小鼠 MCP-5/CCL12(G23-G104)。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

SPR 检测服务   重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
10 μg ¥1000 In-stock
50 μg ¥2500 In-stock
100 μg   询价  

* Please select Quantity before adding items.

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

  • 参考文献

描述

MCP-5/CCL12 protein, Mouse (CHO) is a potent monocyte-active chemokine that binds to the CCR2 chemokine receptor and is involved in allergic inflammation and host responses to pathogens. MCP-5/CCL12 protein, Mouse (CHO) is a recombinant mouse MCP-5/CCL12 (G23-G104) expressed by CHO[1].

研究背景

CCL12,也称为单核细胞趋化蛋白 5 (MCP-5),属于小鼠 11 号染色体上的 CC 趋化因子簇。CCL12 是一种有效的外周血单核细胞趋化因子,可吸引嗜酸性粒细胞、单核细胞和淋巴细胞,但不吸引中性粒细胞。它会诱导外周血单核细胞中的钙流动,但不会诱导纯化的小鼠嗜酸性粒细胞或中性粒细胞中的钙流动。CCL12 是一种有效的单核细胞活性趋化因子,通过 CCR2 发出信号。参与过敏性炎症和宿主对病原体的反应,可能在过敏性肺部炎症的早期阶段发挥关键作用[1][2]

体内研究
(In Vivo)

CCL12 (50 ng/mL) can induce fibroblast migration in wild-type Balb/c mice. However, fibroblasts from CCR2-/- mice are unable to migrate to CCL12. CCL12 expression increases at day 1 after fluorescein isothiocyanate (FITC)-induced fibrosis, peaks at day 3 after FITC, and remains elevated until day 21. And neutralization of CCL12 in wild-type mice significantly prevents FITC-induced fibrosis[3].

生物活性

The ED50 is <0.5 μg/mL as measured by CHO-K1/Gα15/mCCR2 cells (human Gα15 and mouse CCR2 stably expressed in CHO-K1 cells).

种属

Mouse

表达系统

CHO

标签

Tag Free

蛋白编号

Q62401 (G23-G104)

基因 ID

20293  [NCBI]

蛋白结构
N-term
CCL12 (G23-G104)
Accession # Q62401
C-term
同用名
rMuMCP-5/CCL12; C-C motif chemokine 12; MCP5; SCYA12
氨基酸序列

GPDAVSTPVTCCYNVVKQKIHVRKLKSYRRITSSQCPREAVIFRTILDKEICADPKEKWVKNSINHLDKTSQTFILEPSCLG

分子量

9.3-11 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized after extensive dialysis against PBS.

内毒素含量

<0.2 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

MCP-5/CCL12 Protein, Mouse (CHO) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称:

 

* 需求量:

* 客户姓名:

 

* Email:

* 电话:

 

* 公司或机构名称:

   留言给我们:

Bulk Inquiry

Inquiry Information

产品名称:
MCP-5/CCL12 Protein, Mouse (CHO)
目录号:
HY-P7246
需求量: