MCP-5/CCL12 蛋白, Mouse (CHO)

MCP-5/CCL12 protein, Mouse (CHO) is a potent monocyte-active chemokine that binds to the CCR2 chemokine receptor and is involved in allergic inflammation and host responses to pathogens. MCP-5/CCL12 protein, Mouse (CHO) is a recombinant mouse MCP-5/CCL12 (G23-G104) expressed by CHO^[1].

CCL12，也称为单核细胞趋化蛋白 5 (MCP-5)，属于小鼠 11 号染色体上的 CC 趋化因子簇。CCL12 是一种有效的外周血单核细胞趋化因子，可吸引嗜酸性粒细胞、单核细胞和淋巴细胞，但不吸引中性粒细胞。它会诱导外周血单核细胞中的钙流动，但不会诱导纯化的小鼠嗜酸性粒细胞或中性粒细胞中的钙流动。CCL12 是一种有效的单核细胞活性趋化因子，通过 CCR2 发出信号。参与过敏性炎症和宿主对病原体的反应，可能在过敏性肺部炎症的早期阶段发挥关键作用^[1][2]。

CCL12 (50 ng/mL) can induce fibroblast migration in wild-type Balb/c mice. However, fibroblasts from CCR2^-/- mice are unable to migrate to CCL12. CCL12 expression increases at day 1 after fluorescein isothiocyanate (FITC)-induced fibrosis, peaks at day 3 after FITC, and remains elevated until day 21. And neutralization of CCL12 in wild-type mice significantly prevents FITC-induced fibrosis^[3].

The ED₅₀ is <0.5 μg/mL as measured by CHO-K1/Gα15/mCCR2 cells (human Gα15 and mouse CCR2 stably expressed in CHO-K1 cells).

Mouse

CHO

Tag Free

Q62401 (G23-G104)

20293  [NCBI]

GPDAVSTPVTCCYNVVKQKIHVRKLKSYRRITSSQCPREAVIFRTILDKEICADPKEKWVKNSINHLDKTSQTFILEPSCLG

9.3-11 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against PBS.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Sarafi MN,et al. Murine monocyte chemoattractant protein (MCP)-5: a novel CC chemokine that is a structural and functional homologue of human MCP-1. J Exp Med. 1997 Jan 6;185(1):99-109.  [Content Brief]

  [2]. She S, et al. Functional Roles of Chemokine Receptor CCR2 and Its Ligands in Liver Disease. Front Immunol. 2022 Feb 25;13:812431.

  [3]. Bethany B Moore, et al. The role of CCL12 in the recruitment of fibrocytes and lung fibrosis. Am J Respir Cell Mol Biol. 2006 Aug;35(2):175-81.  [Content Brief]