MCP-5/CCL12 蛋白, Mouse (P. pastoris, His)

MCP-5/CCL12 protein promotes CCR2 chemokine receptor binding and contributes to physiological processes such as angiogenesis, inflammation, and monocyte chemotaxis.It is located extracellularly and is widely expressed in tissues such as the central nervous system.MCP-5/CCL12 Protein, Mouse (P.pastoris, His) is the recombinant mouse-derived MCP-5/CCL12 protein, expressed by P.pastoris , with C-His labeled tag.

MCP-5/CCL12 蛋白通过启用 CCR2 趋化因子受体结合活性发挥关键作用，从而促进各种生理过程。它在血管生成、炎症反应和单核细胞趋化性中发挥重要作用。该蛋白预计位于细胞外区域并在细胞外空间活跃，在中枢神经系统和视网膜等组织中表现出广泛的表达模式。该基因的人类直系同源物与多种疾病有关，包括过敏性紫癜、侵袭性牙周炎、自身免疫性疾病、胃肠系统癌症和结核病，强调了其与多种病理状况的潜在参与。与人类 CCL2 和 CCL8 的直系同源关系强调了这种趋化因子蛋白在跨物种中的进化保守性和功能相关性。

Determined by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CCR2A .The ED₅₀ for this effect is 14.24 ng/mL, corresponding to a specific activity is 7.02×10⁴ U/mg.

Mouse

P. pastoris

C-His

NP_035461.2 (G23-G104)

20293  [NCBI]

GPDAVSTPVTCCYNVVKQKIHVRKLKSYRRITSSQCPREAVIFRTILDKEICADPKEKWVKNSINHLDKTSQTFILEPSCLG

Approximately 10.7 kDa.

Greater than 90 % as determined by SDS-PAGE.

无菌粉末.

Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.