Mer 蛋白, Cynomolgus (HEK293, Fc)

Mer protein is a receptor tyrosine kinase that transduces signals by binding to ligands such as LGALS3, TUB, TULP1, or GAS6, regulating cell survival, migration, differentiation, and apoptotic cell phagocytosis (endocytosis). Ligand binding induces autophosphorylation of MERTK, creating a docking site for downstream molecules. Mer Protein, Cynomolgus (HEK293, Fc) is the recombinant cynomolgus-derived Mer protein, expressed by HEK293 , with C-hFc labeled tag. The total length of Mer Protein, Cynomolgus (HEK293, Fc) is 485 a.a., with molecular weight of approximately 150 kDa.

Mer 蛋白是一种受体酪氨酸激酶，通过与各种配体（包括 LGALS3、TUB、TULP1 或 GAS6）结合来转导来自细胞外基质的信号。它参与调节多种生理过程，包括细胞存活、迁移、分化和凋亡细胞的吞噬作用（胞吞作用）。细胞表面的配体结合诱导 MERTK 在其胞内结构域上的自身磷酸化，为下游信号分子创建对接位点。被配体激活后，Mer 与 GRB2 或 PLCG2 相互作用并诱导 MAPK1、MAPK2、FAK/PTK2 或 RAC1 磷酸化。MERTK 信号传导涉及巨噬细胞清除凋亡细胞、血小板聚集、细胞骨架重组和吞噬。在视网膜色素上皮（RPE）中，它充当视杆细胞外节片段吞噬作用的调节器。此外，Mer 在通过激活 STAT1 抑制 Toll 样受体 (TLR) 介导的先天免疫反应中发挥着至关重要的作用，STAT1 选择性诱导细胞因子信号转导 SOCS1 和 SOCS3 抑制剂的产生。

Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Gas6 Protein, His tag at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus MERTK. The ED₅₀ for this effect is 55.09 ng/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Gas6 Protein, His tag at 5μg/mL (100μL/well) can bind Biotinylated Cynomolgus MERTK.The ED₅₀ for this effect is 55.09 ng/mL.

Cynomolgus

HEK293

C-hFc

XP_005575320.1 (A23-I507)

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AITEAREEAKPYPLFPGPLPGSLQTDHTSLLSLPHTSGYQPALMFSPTQPGRPYTGNVAIPRVTSAGSKLLPPLAFKHTVGHIILSEHKDVKFNCSISVPNIYQDTTISWWKDGKELLGAHHAITQFYPDDEVTAIIASFSITSVQRSDNGSYICKMKINNEEIVSDPIYIEVQGLPHFTKQPESMNVTRNTAFNLTCQAVGPPEPVNIFWVQNSSRVNEQPEKSPSVLTVPGLTEMAVFSCEAHNDKGLTVSKGVQINIKAIPSPPTEVSIHNSTAHSILISWVPGFDGYSPFRNCSVQVKEVDPLSNGSVMIFNTSASPHMYQIKQLQALANYSIGVSCMNEIGWSAVSPWILASTTEGAPSVAPLNVTVFLNESRDNVDIRWMKPLTKRQAGELVGYRISHVWQSAGISKELLEEVGQNNSRAQISVQVHNATCTVRIAAVTKGGVGPFSDPVKIFIPAHGWVDHAPSSTPAPGNADPVLII

approximately 150 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.