MERS-CoV Spike/S2 蛋白 (sf9, His)

The SARS-CoV Spike glycoprotein (S) has three subunits S1, S2' and S2 through alternative splicing. The engagement of the MERS-CoV spike protein S1 with CD26 (also known as dipeptidyl peptidase 4, DPP4) mediates viral attachment to host cells and virus鈥揷ell fusion, thereby initiating infection. S2 mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein and S2' is unmasked following S2 cleavage occurring upon virus endocytosis. MERS-CoV Spike/S2 Protein (sf9, His) is the recombinant Virus-derived MERS-CoV Spike/S2 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of MERS-CoV Spike/S2 Protein (sf9, His) is 571 a.a., with molecular weight of ~63.84 kDa.

SARS-CoV 刺突糖蛋白 (S) 通过可变剪切具有 3 个亚基 S1、S2' 和 S2。S1 可以通过与宿主受体的相互作用将病毒粒子附着在细胞膜上，从而引发感染。S2 作为病毒融合蛋白介导病毒粒子与细胞膜的融合。S2' 作为一种病毒融合肽，在病毒内吞作用中发生 S2 裂解后被揭示。
在目前的模型下，S 蛋白至少有三种构象状态:融合前的天然状态、发夹前的中间状态和融合后的发夹状态。在病毒和靶细胞膜融合过程中，卷曲的线圈区域 (七肽重复序列) 呈现三聚发夹结构，使融合肽靠近外胞域的 C 端区域。这种结构的形成似乎驱动了病毒和靶细胞膜的结合和随后的融合。
MERS-CoV 刺突蛋白与 CD26 (也称为二肽基肽酶4,DPP4) 结合介导病毒与宿主细胞的附着和病毒-细胞融合，从而启动感染^[1]。

Virus

Sf9 insect cells

C-His

AFS88936 (D726-P1296)

14254594  [NCBI]

Approximately 63.84 kDa

Greater than 85% as determined by reducing SDS-PAGE

<1 EU/μg, determined by LAL method.