2. 重组蛋白
  3. Receptor Proteins Biotinylated Proteins
  4. Cell Adhesion Molecules (CAMs)
  5. Mesothelin 蛋白, Human (Biotinylated, 286a.a, HEK293, His)

Mesothelin 蛋白, Human (Biotinylated, 286a.a, HEK293, His)

目录号: HY-P77547
产品使用指南

FITC 标记的间皮素蛋白，尤其是其膜锚定形式，可能介导细胞粘附，暗示其在细胞相互作用中的作用。此外，相关的巨核细胞增强因子（MPF）积极增强体外巨核细胞集落的形成，表明其参与促进特化血细胞集落的发育。Mesothelin 蛋白, Human (Biotinylated, 286a.a, HEK293, His) 是重组的 间皮素蛋白，由 HEK293 表达，带有 C-His 标签。Mesothelin 蛋白, Human (Biotinylated, 286a.a, HEK293, His) 全长 286 个氨基酸，分子量约为 ~28.2 KDa。

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描述

FITC-Labeled Mesothelin Protein, especially in its membrane-anchored forms, potentially mediates cellular adhesion, implicating its role in cell interactions. Furthermore, the associated Megakaryocyte-potentiating factor (MPF) actively enhances in vitro megakaryocyte colony formation, indicating its participation in fostering specialized blood cell colonies' development. Mesothelin Protein, Human (Biotinylated, 286a.a, HEK293, His) is the recombinant human-derived Mesothelin protein, expressed by HEK293 , with C-His labeled tag. The total length of Mesothelin Protein, Human (Biotinylated, 286a.a, HEK293, His) is 286 a.a., with molecular weight of ~28.2 KDa.
研究背景

FITC 标记的间皮素蛋白，特别是其膜锚定形式，在细胞粘附中表现出潜在作用，表明参与介导细胞之间的相互作用。此外，相关的巨核细胞增强因子（MPF）已被确定为体外巨核细胞集落形成的有效增强剂，表明其积极参与促进这些特殊血细胞集落的发育。
种属

Human
表达系统

HEK293
标签

C-His
蛋白编号

Q13421-2 (M1-R286)
基因 ID
蛋白结构
N-term
Mesothelin (M1-R286)
Accession # Q13421-2
His
C-term
同用名
Megakaryocyte potentiating factor; mesothelin; CAK1; MPF; MSLN; SMR
分子量

Approximately 28.2 kDa.
纯度

Greater than 95% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

Mesothelin Protein, Human (Biotinylated, 286a.a, HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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