METRNL/Meteorin-like 蛋白, Mouse (His)

METRNL/meteorin-like protein, induced by exercise or cold exposure, promotes energy expenditure. It is produced in skeletal muscle after exercise and in fat tissue after cold exposure and circulates in the body. METRNL/Meteorin-like Protein, Mouse (His) is the recombinant mouse-derived METRNL/Meteorin-like protein, expressed by E. coli , with N-6*His labeled tag.

METRNL/镍纹蛋白样蛋白是一种在运动或寒冷暴露后诱导产生的激素，在促进能量消耗方面发挥作用。它是在运动后的骨骼肌中和寒冷暴露后的脂肪组织中产生的，并且可以在循环中找到。METRNL 通过促进白色脂肪库褐变和改善葡萄糖耐量来刺激能量消耗。有趣的是，它并不直接作用于脂肪细胞以增加产热基因程序，而是刺激各种免疫细胞亚型进入脂肪组织并激活其促产热作用。METRNL 还促进嗜酸性粒细胞依赖性 IL4 表达增加，并促进脂肪组织巨噬细胞的替代激活，这对于脂肪中产热和抗炎基因程序表达的增加至关重要。此外，METRNL 对于某些寒冷诱导的产热反应至关重要，表明它参与了对寒冷温度的代谢适应。

Measured in a cell proliferation assay using SH-SY5Y cells. The ED₅₀ of this effect is 0.193 μg/mL, corresponding to a specific activity is 5.18×10³ units/mg.

• 
  Measured in a cell proliferation assay using SH-SY5Y cells.  The ED₅₀ for this effect is 0.193 μg/mL, corresponding to a specific activity is 5.18×10³ units/mg.

Mouse

E. coli

N-6*His

Q8VE43-1 (Q46-E311)

210029  [NCBI]

QYSSDLCSWKGSGLTREARSKEVEQVYLRCSAGSVEWMYPTGALIVNLRPNTFSPAQNLTVCIKPFRDSSGANIYLEKTGELRLLVRDIRGEPGQVQCFSLEQGGLFVEATPQQDISRRTTGFQYELMSGQRGLDLHVLSAPCRPCSDTEVLLAICTSDFVVRGFIEDVTHVPEQQVSVIYLRVNRLHRQKSRVFQPAPEDSGHWLGHVTTLLQCGVRPGHGEFLFTGHVHFGEAQLGCAPRFSDFQRMYRKAEEMGINPCEINME

30-33 kDa

• 
  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, 6% Trehalose, pH 7.4 or PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.