MIA 蛋白, Human (N-His)

MIA proteins exhibit significant growth inhibitory effects on melanoma cells in vitro, extending their effects to various neuroectodermal tumors such as gliomas. In addition to playing an inhibitory role in cell growth, MIA proteins are also involved in molecular interactions, interacting with FASLG and TMIGD2. MIA Protein, Human (N-His) is the recombinant human-derived MIA protein, expressed by E. coli , with N-6*His labeled tag. The total length of MIA Protein, Human (N-His) is 107 a.a., with molecular weight of ~14.0 kDa.

黑色素瘤抑制活性 (MIA) 是一种蛋白质，因其在体外对黑色素瘤细胞具有生长抑制作用而闻名，并且对各种神经外胚层肿瘤（例如神经胶质瘤）也显示出类似的作用。MIA 引起生长抑制的能力表明其作为肿瘤抑制因子的潜在作用，特别是在源自神经外胚层组织的恶性肿瘤的情况下。此外，MIA 与 FASLG 相互作用，表明它参与与细胞凋亡和免疫反应相关的细胞过程。此外，它与 TMIGD2 的相互作用表明其在调节信号通路或细胞粘附方面具有潜在作用。MIA 的多方面相互作用和生长抑制特性凸显了其在肿瘤生长调节中的重要性及其在神经外胚层癌中的潜在治疗意义。

Measured in a cell proliferation assay using human A375 melanoma cell line.The ED₅₀ for this effect is 4-8 μg/mL, corresponding to a specific activity is 135-244 units/mg.

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  Measured in a cell proliferation assay using human A375 melanoma cell line.The ED₅₀ for this effect is 4-8 μg/mL, corresponding to a specific activity is 135-244 units/mg.

Human

E. coli

N-6*His

Q16674 (G25-Q131)

8190  [NCBI]

GPMPKLADRKLCADQECSHPISMAVALQDYMAPDCRFLTIHRGQVVYVFSKLKGRGRLFWGGSVQGDYYGDLAARLGYFPSSIVREDQTLKPGKVDVKTDKWDFYCQ

Approximately 14.0 kDa

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  Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, 200 mM arginine, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.