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  3. MID1IP1 蛋白, Human (His)

MID1IP1 蛋白, Human (His)

目录号: HY-P76497
COA 产品使用指南

Mid1-interacting 蛋白 1 在调节肝脏脂肪生成中发挥作用,并上调 ACACA 酶活性。Mid1-interacting 蛋白 1 是高效脂质生物合成(包括三酰甘油、二酰甘油和磷脂)所必需的。Mid1-interacting 蛋白 1 参与微管的稳定。MID1IP1 蛋白, Human (His) 是重组的 MID1IP1 蛋白,由 E. coli 表达,带有 N-His 标签。MID1IP1 蛋白, Human (His) 全长 183 个氨基酸,分子量约为 ~28 KDa。

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

Mid1-interacting protein 1 plays a role in the regulation of lipogenesis in liver and up-regulates ACACA enzyme activity. Mid1-interacting protein 1 required for efficient lipid biosynthesis, including triacylglycerol, diacylglycerol and phospholipid. Mid1-interacting protein 1 involved in stabilization of microtubules. MID1IP1 Protein, Human (His) is the recombinant human-derived MID1IP1 protein, expressed by E. coli , with N-His labeled tag. The total length of MID1IP1 Protein, Human (His) is 183 a.a., with molecular weight of ~25 KDa.

研究背景

Mid1-interacting protein 1 具有相同的蛋白结合活性和蛋白c末端结合活性。Mid1-interacting protein 1 参与多个过程,包括微管解聚的负调控;脂肪酸生物合成过程的正向调控;以及蛋白质聚合。Mid1-interacting protein 1 定位于细胞质和微管细胞骨架,在细胞质中具有活性[1][2]

种属

Human

表达系统

E. coli

标签

N-6*His

蛋白编号

Q9NPA3 (M1-H183)

基因 ID
蛋白结构
N-term
His
MID1IP1 (M1-H183)
Accession # Q9NPA3
C-term
同用名
Mid1-interacting protein 1; Protein STRAIT11499; Spot 14-related protein; S14R; MIG12
氨基酸序列

MMQICDTYNQKHSLFNAMNRFIGAVNNMDQTVMVPSLLRDVPLADPGLDNDVGVEVGGSGGCLEERTPPVPDSGSANGSFFAPSRDMYSHYVLLKSIRNDIEWGVLHQPPPPAGSEEGSAWKSKDILVDLGHLEGADAGEEDLEQQFHYHLRGLHTVLSKLTRKANILTNRYKQEIGFGNWGH

分子量

Approximately 25 kDa.

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4, 10% Glycerol.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

MID1IP1 Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
MID1IP1 Protein, Human (His)
目录号:
HY-P76497
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