1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. MME 蛋白, Human (HEK293)

MME 蛋白, Human (HEK293)

目录号: HY-P72876
COA 产品使用指南

MME 蛋白表现出类似嗜热菌蛋白酶的特异性,主要降解最多 30 个氨基酸的多肽。至关重要的是,它在裂解 Gly-Phe 键以降解阿片肽(包括甲硫氨酸脑啡肽和亮氨酸脑啡肽)方面发挥着关键作用。MME 蛋白, Human (HEK293) 是重组的 MME 蛋白,由 HEK293 表达,不带标签。MME 蛋白, Human (HEK293) 全长 699 个氨基酸,分子量为 80-100 kDa。

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规格 价格 是否有货 数量
5 μg ¥800 In-stock
10 μg ¥1360 In-stock
50 μg ¥3800 In-stock
100 μg ¥6460 询价
> 100 μg   询价  

* Please select Quantity before adding items.

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

MME proteins exhibit thermolysin-like specificity and primarily degrade polypeptides of up to 30 amino acids. Crucially, it plays a key role in cleaving the Gly-Phe bond to degrade opioid peptides, including Met-enkephalin and Leu-enkephalin. MME Protein, Human (HEK293) is the recombinant human-derived MME protein, expressed by HEK293 , with tag free. The total length of MME Protein, Human (HEK293) is 699 a.a., with molecular weight of 80-100 kDa.

研究背景

MME 蛋白表现出类似嗜热菌蛋白酶的特异性,主要作用于最多 30 个氨基酸的多肽。在生物学上至关重要,它在阿片肽(包括甲硫氨酸和亮氨酸脑啡肽)的降解中发挥着关键作用,这是通过 Gly-Phe 键的裂解实现的。此外,MME 催化缓激肽、P 物质和神经降压素肽的裂解,并且能够裂解血管紧张素-1、血管紧张素-2 和血管紧张素 1-9。该蛋白参与心房钠尿因子 (ANF) 和脑钠尿因子 (BNP(1-32)) 的降解。此外,MME 对皮肤预弹性纤维和弹性纤维表现出紫外线诱导的弹性蛋白酶活性。

生物活性

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK (Dnp)-OH (HY-P3749). The specific activity is > 1500 pmole/min/μg.

种属

Human

表达系统

HEK293

标签

Tag Free

蛋白编号

P08473 (Y52-W750)

基因 ID
蛋白结构
N-term
MME (Y52-W750)
Accession # P08473
C-term
同用名
Neprilysin; Atriopeptidase; CALLA; NEP; SFE; CD10; MME; EPN
氨基酸序列

YDDGICKSSDCIKSAARLIQNMDATTEPCTDFFKYACGGWLKRNVIPETSSRYGNFDILRDELEVVLKDVLQEPKTEDIVAVQKAKALYRSCINESAIDSRGGEPLLKLLPDIYGWPVATENWEQKYGASWTAEKAIAQLNSKYGKKVLINLFVGTDDKNSVNHVIHIDQPRLGLPSRDYYECTGIYKEACTAYVDFMISVARLIRQEERLPIDENQLALEMNKVMELEKEIANATAKPEDRNDPMLLYNKMTLAQIQNNFSLEINGKPFSWLNFTNEIMSTVNISITNEEDVVVYAPEYLTKLKPILTKYSARDLQNLMSWRFIMDLVSSLSRTYKESRNAFRKALYGTTSETATWRRCANYVNGNMENAVGRLYVEAAFAGESKHVVEDLIAQIREVFIQTLDDLTWMDAETKKRAEEKALAIKERIGYPDDIVSNDNKLNNEYLELNYKEDEYFENIIQNLKFSQSKQLKKLREKVDKDEWISGAAVVNAFYSSGRNQIVFPAGILQPPFFSAQQSNSLNYGGIGMVIGHEITHGFDDNGRNFNKDGDLVDWWTQQSASNFKEQSQCMVYQYGNFSWDLAGGQHLNGINTLGENIADNGGLGQAYRAYQNYIKKNGEEKLLPGLDLNHKQLFFLNFAQVWCGTYRPEYAVNSIKTDVHSPGNFRIIGTLQNSAEFSEAFHCRKNSYMNPEKKCRVW

分子量

80-100 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

溶液

组分

Supplied as a 0.22 μm filtered solution in 50 mM MES, 100 mM NaCl, 10% glycerol, pH 6.5.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

N/A.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料

MME Protein, Human (HEK293) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
MME Protein, Human (HEK293)
目录号:
HY-P72876
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