MME 蛋白, Human (P.pastoris, His)

MME proteins exhibit thermolysin-like specificity and primarily degrade polypeptides of up to 30 amino acids. Crucially, it plays a key role in cleaving the Gly-Phe bond to degrade opioid peptides, including Met-enkephalin and Leu-enkephalin. MME Protein, Human (P.pastoris, His) is the recombinant human-derived MME protein, expressed by P. pastoris , with N-His labeled tag. The total length of MME Protein, Human (P.pastoris, His) is 699 a.a., with molecular weight of ~81.8 kDa.

MME 蛋白表现出类似嗜热菌蛋白酶的特异性，主要作用于最多 30 个氨基酸的多肽。在生物学上至关重要，它在阿片肽（包括甲硫氨酸和亮氨酸脑啡肽）的降解中发挥着关键作用，这是通过 Gly-Phe 键的裂解实现的。此外，MME 催化缓激肽、P 物质和神经降压素肽的裂解，并且能够裂解血管紧张素-1、血管紧张素-2 和血管紧张素 1-9。该蛋白参与心房钠尿因子 (ANF) 和脑钠尿因子 (BNP(1-32)) 的降解。此外，MME 对皮肤预弹性纤维和弹性纤维表现出紫外线诱导的弹性蛋白酶活性。

Human

P. pastoris

N-His

P08473 (Y52-W750)

4311  [NCBI]

YDDGICKSSDCIKSAARLIQNMDATTEPCTDFFKYACGGWLKRNVIPETSSRYGNFDILRDELEVVLKDVLQEPKTEDIVAVQKAKALYRSCINESAIDSRGGEPLLKLLPDIYGWPVATENWEQKYGASWTAEKAIAQLNSKYGKKVLINLFVGTDDKNSVNHVIHIDQPRLGLPSRDYYECTGIYKEACTAYVDFMISVARLIRQEERLPIDENQLALEMNKVMELEKEIANATAKPEDRNDPMLLYNKMTLAQIQNNFSLEINGKPFSWLNFTNEIMSTVNISITNEEDVVVYAPEYLTKLKPILTKYSARDLQNLMSWRFIMDLVSSLSRTYKESRNAFRKALYGTTSETATWRRCANYVNGNMENAVGRLYVEAAFAGESKHVVEDLIAQIREVFIQTLDDLTWMDAETKKRAEEKALAIKERIGYPDDIVSNDNKLNNEYLELNYKEDEYFENIIQNLKFSQSKQLKKLREKVDKDEWISGAAVVNAFYSSGRNQIVFPAGILQPPFFSAQQSNSLNYGGIGMVIGHEITHGFDDNGRNFNKDGDLVDWWTQQSASNFKEQSQCMVYQYGNFSWDLAGGQHLNGINTLGENIADNGGLGQAYRAYQNYIKKNGEEKLLPGLDLNHKQLFFLNFAQVWCGTYRPEYAVNSIKTDVHSPGNFRIIGTLQNSAEFSEAFHCRKNSYMNPEKKCRVW

Approximately 81.8 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.