Stromelysin-1/MMP-3 蛋白, Human

The Stromelysin-1/MMP-3 protein is a multifunctional metalloprotease that degrades a variety of extracellular matrix components and activates molecules such as growth factors, plasminogen, and MMP9. It is released into the ECM and is activated through the plasmin cascade. Stromelysin-1/MMP-3 Protein, Human is the recombinant human-derived Stromelysin-1/MMP-3 protein, expressed by E. coli , with tag free. The total length of Stromelysin-1/MMP-3 Protein, Human is 255 a.a., with molecular weight of ~34 kDa.

Stromelysin-1/MMP-3 是一种金属蛋白酶，具有广泛的底物特异性，能够降解细胞外基质 (ECM) 的各种成分，例如纤连蛋白、层粘连蛋白、明胶（I、III、IV 和 V 型）、胶原蛋白（ III、IV、X 和 IX) 和软骨蛋白聚糖。这种酶在激活不同分子中发挥着关键作用，包括生长因子、纤溶酶原或其他基质金属蛋白酶（如 MMP9）。释放到 ECM 后，无活性的酶原通过纤溶酶级联信号通路进行激活。Stromelysin-1/MMP-3 也在细胞内发挥作用，正如在多巴胺能神经元中观察到的那样，在应激过程中，它被丝氨酸蛋白酶 HTRA2 激活，通过介导小胶质细胞激活和 α-突触核蛋白/SNCA 裂解，导致多巴胺神经元变性。此外，这种金属蛋白酶在免疫反应中发挥作用，并对多种病毒表现出抗病毒活性，包括水泡性口炎病毒、甲型流感病毒 (H1N1) 和人类疱疹病毒 1。从机制上讲，它在病毒感染时从细胞质转位到细胞核中调节 NF-kappa-B 活性。

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nva-WR-K(Dnp)-NH2 and the specific activity is >300 pmoles/min/μg. (Activation description: The proenzyme needs to be activated by Chymotrypsin for an activated form).

Human

E. coli

Tag Free

P08254 (Y18-T272)

4314  [NCBI]

YPLDGAARGEDTSMNLVQKYLENYYDLKKDVKQFVRRKDSGPVVKKIREMQKFLGLEVTGKLDSDTLEVMRKPRCGVPDVGHFRTFPGIPKWRKTHLTYRIVNYTPDLPKDAVDSAVEKALKVWEEVTPLTFSRLYEGEADIMISFAVREHGDFYPFDGPGNVLAHAYAPGPGINGDAHFDDDEQWTKDTTGTNLFLVAAHEIGHSLGLFHSANTEALMYPLYHSLTDLTRFRLSQDDINGIQSLYGPPPDSPET

Approximately 34 kDa

Greater than 90% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 10 mM CaCL₂, 1uM ZnCL₂, 50 mM NaCl, 0.5% Brij35, pH 7.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Less than 1 EU/µg as determined by LAL test.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.